Mugla Sitki Kocman University, Faculty of Science, Chemistry Department, Kotekli/Mugla, Turkey.
Analyst. 2017 Dec 18;143(1):150-156. doi: 10.1039/c7an01537b.
An effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10 U mL and 10 U mL was found with a relative standard deviation value of 3.23% (for 10 U mL of N, n:3) and a limit of detection value of 10 U mL N. The developed biosensor was applied for real influenza virus A (H9N2) detection and very successful results were obtained.
基于石墨烯-金（Au）杂化纳米复合材料修饰的 Au 丝网印刷电极，开发了一种有效的电化学流感 A 生物传感器。所开发的生物传感器的工作原理依赖于神经氨酸酶（N）活性的测量。在优化实验参数（如牛血清白蛋白的添加效果和胎球蛋白 A 和 PNA 凝集素的固定时间）后，研究了流感 A 生物传感器的分析特性。结果，发现线性范围在 10 U mL 至 10 U mL 之间，相对标准偏差值为 3.23%（N 为 10 U mL，n：3），检测限值为 10 U mL N。所开发的生物传感器用于实际流感病毒 A（H9N2）的检测，并获得了非常成功的结果。
