Chmelař Dittmar, Kašíková Adéla, Martineková Petrana, Hájek Michal, Rozložník Miroslav, Brabec Marek, Janečková Jana, Vobejdová Jana, Čižnár Ivan
Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00, Ostrava-Zabreh, Czech Republic.
Czech Anaerobic Bacteria Reference Laboratory, Faculty of Medicine, University of Ostrava, Ostrava-Zabreh, Czech Republic.
Folia Microbiol (Praha). 2018 May;63(3):283-290. doi: 10.1007/s12223-017-0564-1. Epub 2017 Nov 13.
The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses. Endotoxin production was proven by quantitative detection using the limulus amebocyte lysate (LAL) test in EU/mL. Production of endotoxins in these bacteria cultured under hyperbaric air conditions was higher than those strains cultured under normobaric anaerobic conditions. But these differences in endotoxin production were not statistically significant (t test with log-transformed data, p value = 0.0910). Based on a two-tier t test for lognormal data, it is possible to cautiously conclude that a statistically significant difference was found between endotoxin production by Bacteroides fragilis strains isolated from non-carcinoma diagnoses (strains (1-6) and strains isolated from colorectal carcinoma diagnoses (strains 7-8; Wilcoxon non-parametric test p = 0.0132; t test = 0.1110; t test with log-transformed data, p value = 0.0294).
该项目的目的是确定高压空气对在高压条件下培养的脆弱拟杆菌菌株的任何影响。此前，有人假设，偏爱肉食的患者体内存在拟杆菌与小肠癌，尤其是大肠和直肠癌之间存在关联。鉴于脆弱拟杆菌（BAFR）群是内毒素的重要产生者，通过对分离出的单个拟杆菌菌株的内毒素产生情况进行测量和统计评估，来比较从结肠癌和直肠癌患者的各种临床样本中分离出的细菌与从其他非癌症诊断中分离出的菌株。使用鲎试剂（LAL）试验以EU/mL为单位进行定量检测，证明了内毒素的产生。在高压空气条件下培养的这些细菌中内毒素的产生高于在常压厌氧条件下培养的菌株。但这些内毒素产生的差异无统计学意义（对数据进行对数转换后的t检验，p值 = 0.0910）。基于对对数正态数据的两层t检验，可以谨慎地得出结论，从非癌诊断中分离出的脆弱拟杆菌菌株（菌株1 - 6）与从结直肠癌诊断中分离出的菌株（菌株7 - 8）在内毒素产生方面存在统计学显著差异（Wilcoxon非参数检验p = 0.0132；t检验 = 0.1110；对数据进行对数转换后的t检验，p值 = 0.0294）。
