用对流免疫电泳法检测羊水脆弱拟杆菌内毒素
Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis.
作者信息
Beckmann I, de Graaff K, Meisel-Mikołajczyk F, Wallenburg H C
机构信息
Department of Obstetrics and Gynecology, Erasmus University Medical School, Rotterdam, The Netherlands.
出版信息
Antonie Van Leeuwenhoek. 1994;66(4):333-6. doi: 10.1007/BF00882769.
The ability of counter immunoelectrophoresis (CIE) to detect Bacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection of B. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.
评估了对流免疫电泳（CIE）检测羊水中小浓度脆弱拟杆菌内毒素的能力。开发了一种方法，该方法与超滤相结合，能够检测浓度为40 ng/ml或更高的羊水中的脆弱拟杆菌内毒素。通过使用从兔抗脆弱拟杆菌IPL E 323抗血清中分离出的高反应性IgG组分，灵敏度阈值降低到了2 ng/ml。
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