直接从单个噬菌斑对含双链DNA的噬菌体进行基因组测序。
Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque.
作者信息
Kot Witold
机构信息
Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark.
出版信息
Methods Mol Biol. 2018;1681:179-184. doi: 10.1007/978-1-4939-7343-9_13.
PMID:29134595
Abstract
The sequencing of phage genomes has become a routine procedure for phage characterization. The protocol presented here allows rapid isolation of DNA from a single phage plaque followed by building ready-to-sequence Illumina-compatible library.
摘要
噬菌体基因组测序已成为噬菌体特性鉴定的常规程序。本文介绍的方案可从单个噬菌斑中快速分离DNA，随后构建适用于Illumina测序的文库。
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