Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy.
Department of Veterinary Medicine, University of Bari, Bari, Italy.
Transbound Emerg Dis. 2018 Feb;65(1):16-21. doi: 10.1111/tbed.12747. Epub 2017 Nov 13.
Canine parvovirus (CPV) is an important infectious agent of domestic and wild carnivores, responsible for severe and often fatal haemorrhagic gastroenteritis and leukopenia. This paper reports the genomic characterization of a CPV strain collected from a dog recently imported to Italy from Thailand. The virus was detected in all tissue samples collected. The whole genome encompassing the two open reading frames encoding for non-structural (NS1/NS2) and structural (VP1/VP2) proteins was amplified and sequenced. On the basis of genetic analysis of the VP2 gene, the isolate was characterized as CPV-2c, but it presented genetic signatures typical of Asian strains. Sequence analysis revealed the presence of amino acid changes never observed in European CPV-2c strains (NS1: Ile60Val, Tyr544Phe, Glu545Val, Leu630Pro; VP2: Ala5Gly, Phe267Tyr, Tyr324Ile, Gln370Arg). By phylogenetic analysis of full-length VP2 gene, the analysed strain clustered together with Asian viruses. Therefore, a possible introduction of the virus from Asia through the imported dog was suggested, thus confirming the important role of movement of dogs in the global spread of viruses. In addition, full-length genome analysis could help better trace the spread of canine viruses through different continents.
犬细小病毒（CPV）是一种重要的感染性病原体，可导致家养和野生食肉动物发生严重且常致命的出血性胃肠炎和白细胞减少症。本文报告了从最近从泰国进口到意大利的一只狗身上采集的 CPV 毒株的基因组特征。在采集的所有组织样本中均检测到了该病毒。扩增并测序了包含编码非结构（NS1/NS2）和结构（VP1/VP2）蛋白的两个开放阅读框的全基因组。根据 VP2 基因的遗传分析，该分离株被鉴定为 CPV-2c，但具有亚洲株的遗传特征。序列分析显示存在从未在欧洲 CPV-2c 株中观察到的氨基酸变化（NS1：Ile60Val、Tyr544Phe、Glu545Val、Leu630Pro；VP2：Ala5Gly、Phe267Tyr、Tyr324Ile、Gln370Arg）。通过全长 VP2 基因的系统进化分析，分析的毒株与亚洲病毒聚集在一起。因此，提示该病毒可能是通过进口犬从亚洲传入，这证实了犬只在病毒的全球传播中起着重要作用。此外，全长基因组分析有助于更好地追踪不同大陆之间犬病毒的传播。
