Popoola Bosede, Ashefor Olivia, Akanni Olubukola, Adaramoye Oluwatosin
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan.
Niger J Physiol Sci. 2017 Jun 30;32(1):75-84.
Diabetes mellitus (DM) is characterized by hyperglycemia and endocrine disorder. Diabetes mellitus is knownto promote male infertility by affecting sperm quality through altered steroidogenesis. The role of prostatic fluid in maintenance of sperm quality have been established. However, the effect of DM on prostate health is poorly understood.This study was designed to investigate the biochemical, hormonal and histological changes in prostate of male Wistar ratsafter 3- and 5-months DM. Twenty-six adult male Wistar rats were assigned into three groups. Group I (non-diabetic rats)served as control (n=10), out of which five rats (n=5) were sacrificed as control for 3-month study and the remaining animals(n=5) as control for 5-month study. Group II served as 3-month DM (n=8) and group III served as 5-month DM (n=8).Diabetes mellitus was induced by administration of a single dose of streptozotocin (STZ) (35 mg/kg i.p.). Rats were sacrificedat 3- and 5- months after DM. Biochemical indices in serum and prostate, histological and immunohistochemical studies ofthe prostate were evaluated. Results indicated that the weight of prostate of 3- and 5-months DM rats significantly (p<0.05)decreased by 33% and 59%, respectively. Fasting blood glucose, plasma glycated haemoglobin and number ofmicronucleated polychromatic erythrocytes in the bone marrow significantly (p<0.05) increased in 3- and 5-months DM.Activities of serum alanine and aspartate aminotransferases significantly (p<0.05) increased in 3 months DM withconcomitant increase in serum total bilirubin and urea in both models. The activities of total acid phosphatase in 3- and 5-months DM decreased by 34% and 76%, respectively while prostatic acid phosphatase decreased by 48% in 5-months DM.Prostatic zinc and bicarbonate increased in 3-months DM by 1.7 and 1.9 folds, and in 5-months DM by 5.8 and 1.7 folds,respectively. The levels of serum luteinizing and follicle stimulating hormones and testosterone decreased in both models.Prostatic lipid peroxidation increased while activities of antioxidative enzymes decreased in both models. Histology revealedhyperplasia, intra-luminal budding of epithelia, mild expressions of Bcl2 and Ki67 in 3- and 5-months DM. Overall, prostate health was compromised and may increase infertility in diabetic animals.
糖尿病（DM）的特征为高血糖和内分泌紊乱。已知糖尿病通过改变类固醇生成影响精子质量，从而导致男性不育。前列腺液在维持精子质量方面的作用已得到证实。然而，糖尿病对前列腺健康的影响却知之甚少。本研究旨在调查雄性Wistar大鼠在患糖尿病3个月和5个月后前列腺的生化、激素和组织学变化。26只成年雄性Wistar大鼠被分为三组。第一组（非糖尿病大鼠）作为对照组（n = 10），其中5只大鼠（n = 5）作为3个月研究的对照组被处死，其余动物（n = 5）作为5个月研究的对照组。第二组作为3个月糖尿病组（n = 8），第三组作为5个月糖尿病组（n = 8）。通过腹腔注射单剂量链脲佐菌素（STZ）（35 mg/kg）诱导糖尿病。在糖尿病发生后的3个月和5个月处死大鼠。评估血清和前列腺中的生化指标、前列腺的组织学和免疫组化研究。结果表明，3个月和5个月糖尿病大鼠的前列腺重量分别显著（p<0.05）下降了33%和59%。3个月和5个月糖尿病大鼠的空腹血糖、血浆糖化血红蛋白和骨髓中微核多染红细胞数量显著（p<0.05）增加。3个月糖尿病大鼠血清丙氨酸和天冬氨酸转氨酶活性显著（p<0.05）增加，且两种模型中血清总胆红素和尿素均随之增加。3个月和5个月糖尿病大鼠的总酸性磷酸酶活性分别下降了34%和76%，而5个月糖尿病大鼠的前列腺酸性磷酸酶下降了48%。3个月糖尿病大鼠前列腺锌和碳酸氢盐分别增加了1.7倍和1.9倍，5个月糖尿病大鼠分别增加了5.8倍和1.7倍。两种模型中血清黄体生成素、卵泡刺激素和睾酮水平均下降。两种模型中前列腺脂质过氧化增加，抗氧化酶活性下降。组织学显示，3个月和5个月糖尿病大鼠出现增生、上皮腔内芽生、Bcl2和Ki67轻度表达。总体而言，前列腺健康受到损害，可能会增加糖尿病动物的不育率。
