Biology Department, College of Education for Pure Science, University of Thi-Qar, Nasiriyah, Iraq.
Pharmacology Department, College of Pharmacy, Al-Muthanna University, Samawah, Iraq.
Arch Razi Inst. 2022 Apr 30;77(2):907-913. doi: 10.22092/ARI.2022.357126.1982. eCollection 2022 Apr.
Prostate dysfunction is the most common condition among aged men, which causes adverse complications and may result in serious diseases. has been studied since time immemorial in several studies all showing its ability in preventing and treating different diseases. However, so far there have been no studies focusing on the possible role of in the protection of prostate histoarchitecture toxicity. Therefore, this study aimed to investigate the protective role of in the amelioration of histological and hormonal depression affected by sodium fluoride (NaF). A total of 28 male adult Wistar rats were equally divided into four groups (n=7). Animals in the control group received normal saline. The second group received NaF by oral gavage at a dose of 12 mg/kg body weight (B.W.) three times a week. The third group received concurrent treatment with NaF at a dose of 12 mg/kg B.W. three times a week, as well as extraction of at a dose of 100 mg/kg B.W. The fourth group was treated only with extraction of at a dose of 100 mg/k B.W. After 60 days, B.W. and the absolute weight of the prostate were measured. Blood samples and tissues were collected for measuring testosterone, follicle-stimulating hormone, as well as luteinizing hormone concentration, conducting paraffin-embedded sections with hematoxylin, and eosin routine staining. The findings revealed that supplement significantly increased body and absolute weight of prostate gland in the group treated by NaF. In addition, mitigating the histological changes throughout the restoration of all prostate components appeared nearly as normal structural tissue. Moreover, the height of glandular epithelium decreased, follicular lumen enlarged, dark secretion materials with homogeneity disappeared of invagination intraluminal, and normal stroma appeared in regular shape. All in all, the results of this study pointed out that had a protective effect against NaF-influenced prostate toxicity via stabilizing male hormones, re-composing histoarchitecture, and returning abnormal biomorphic parameters to a nearly normal state.
前列腺功能障碍是老年男性最常见的病症，会引起不良并发症，甚至可能导致严重疾病。自古以来，人们就在多项研究中对其进行研究，这些研究均表明它具有预防和治疗各种疾病的能力。然而，迄今为止，还没有研究关注 对保护前列腺组织学结构免受氟化物毒性的可能作用。因此，本研究旨在探讨 对改善氟化物（NaF）影响的组织学和激素抑制的保护作用。总共 28 只成年雄性 Wistar 大鼠被平均分为四组（n=7）。对照组动物接受生理盐水。第二组通过口服灌胃给予 12mg/kg 体重（B.W.）的 NaF，每周三次。第三组同时给予 12mg/kg B.W.的 NaF，每周三次，以及 100mg/kg B.W.的 提取物。第四组仅用 100mg/kg B.W.的 提取物治疗。60 天后，测量 B.W.和前列腺的绝对重量。采集血液样本和组织，以测量睾酮、卵泡刺激素和黄体生成素浓度，进行苏木精-伊红常规染色的石蜡包埋切片。研究结果表明，在 NaF 处理组中， 补充剂显著增加了 B.W.和前列腺的绝对重量。此外，通过恢复所有前列腺成分，减轻了组织学变化，组织结构几乎恢复正常。此外，腺上皮高度降低，滤泡腔增大，均匀消失的深分泌物质内陷，正常基质呈规则形状。总的来说，这项研究的结果表明， 对 NaF 影响的前列腺毒性具有保护作用，通过稳定雄性激素、重新组成组织学结构以及将异常生物形态参数恢复到接近正常状态。
