Fagundes Danny L G, França Eduardo L, Gonzatti Michelangelo B, Rugde Marilza V C, Calderon Iracema M P, Honorio-França Adenilda C
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University/Unesp, São Paulo, SP, Brazil.
Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.
APMIS. 2018 Jan;126(1):56-64. doi: 10.1111/apm.12772. Epub 2017 Nov 14.
The study investigated the role of cytokines IL-4 and IL-17 in the modulation of the functional activity of mononuclear phagocytes in diabetic pregnant women with hyperglycemia. Sixty pregnant women were assigned to the following groups: nondiabetic (ND), mild gestational hyperglycemia (MGH), gestational diabetes mellitus (GDM), or type 2 diabetes mellitus (DM2). The functional activity of phagocytes from maternal blood, cord blood, and colostrum was assessed by determining their superoxide release, phagocytosis, microbicidal activity, and intracellular Ca release. Irrespective of glycemic status, colostrum and blood cells treated with IL-4 and IL-17 increased superoxide release in the presence of enteropathogenic Escherichia coli (EPEC). The highest phagocytosis rate was observed in cells from the DM2 group treated with IL-4. In all the groups, phagocytes from colostrum, maternal blood, and cord blood exhibited higher microbicidal activity against EPEC when treated with cytokines. IL-17 increased intracellular Ca release by colostrum phagocytes in diabetic groups. The results indicate that the IL-4 and IL-17 modulate the functional activity of phagocytes in the maternal blood, cord blood, and colostrum of diabetic mother. The natural immunity resulting from the interaction between the cells and cytokines tested may be an alternative procedure to improve the prognosis of maternal and newborn infections.
该研究调查了细胞因子白细胞介素-4（IL-4）和白细胞介素-17（IL-17）在调节高血糖糖尿病孕妇单核吞噬细胞功能活性中的作用。60名孕妇被分为以下几组：非糖尿病组（ND）、轻度妊娠期高血糖组（MGH）、妊娠期糖尿病组（GDM）或2型糖尿病组（DM2）。通过测定其超氧化物释放、吞噬作用、杀菌活性和细胞内钙释放来评估母体血液、脐带血和初乳中吞噬细胞的功能活性。无论血糖状态如何，用IL-4和IL-17处理的初乳和血细胞在存在肠致病性大肠杆菌（EPEC）的情况下超氧化物释放增加。在用IL-4处理的DM2组细胞中观察到最高的吞噬率。在所有组中，用细胞因子处理时，初乳、母体血液和脐带血中的吞噬细胞对EPEC表现出更高的杀菌活性。IL-17增加了糖尿病组初乳吞噬细胞的细胞内钙释放。结果表明，IL-4和IL-17调节糖尿病母亲的母体血液、脐带血和初乳中吞噬细胞的功能活性。所测试的细胞与细胞因子之间相互作用产生的天然免疫可能是改善母婴感染预后的一种替代方法。
