DNA聚合酶使人工核苷酸选择性掺入与DNA加合物相对的位置的结构基础。
Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
作者信息
Betz K, Nilforoushan A, Wyss L A, Diederichs K, Sturla S J, Marx A
机构信息
Department of Chemistry & Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstr. 10, D-78457 Konstanz, Germany.
出版信息
Chem Commun (Camb). 2017 Nov 23;53(94):12704-12707. doi: 10.1039/c7cc07173f.
The possibility to sequence cytotoxic O-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing.
对细胞毒性O-烷基鸟嘌呤DNA加合物进行测序的可能性将极大地推动研究进展。最近，我们报道了一种苯并咪唑衍生的核苷酸，它能被一种突变的DNA聚合酶选择性地掺入到损伤位点的对面。在此，我们提供了这一反应的结构基础，这可能会促进DNA损伤测序的未来发展。
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