School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Biosens Bioelectron. 2018 Apr 15;102:189-195. doi: 10.1016/j.bios.2017.11.032. Epub 2017 Nov 9.
A sensitive sandwich-type electrochemical immunosensor for the detection of hepatitis B e antigen (HBeAg) was successfully developed based on the gold@palladium nanoparticles (Au@Pd NPs) loaded by molybdenum disulfide functionalized multiwalled carbon nanotubes (Au@Pd/MoS@MWCNTs). The resultant nanocomposites not only possessed high specific surface area and good biocompatibility, but also exhibited excellent electro-catalytical property. Au NPs functionalized porous graphene oxide (p-GO@Au) were used as sensing platforms and primary antibodies carriers, which can accelerate the electron transfer and improve the load capacity of primary antibodies (Ab), improving the sensitivity of the immunosensor. Under optimal conditions, the designed immunosensor could detect target HBeAg concentration in the range from 0.1pg/mL to 500pg/mL, with a low detection limit of 26fg/mL (S/N = 3) for HBeAg. Additionally, the designed immunosensor showed excellent specificity, good reproducibility and acceptable stability. The satisfactory results in analysis of human serum samples indicated that it had potential application in clinical monitoring of tumor markers.
基于负载二硫化钼功能化多壁碳纳米管的金钯纳米粒子（Au@Pd NPs），成功研制出一种用于检测乙型肝炎 e 抗原（HBeAg）的灵敏三明治型电化学免疫传感器。所得纳米复合材料不仅具有高比表面积和良好的生物相容性，而且表现出优异的电催化性能。功能化多孔氧化石墨烯（p-GO@Au）上的金纳米粒子（Au NPs）用作传感平台和一级抗体载体，可加速电子转移并提高一级抗体（Ab）的负载量，从而提高免疫传感器的灵敏度。在最佳条件下，设计的免疫传感器可以检测 0.1pg/mL 至 500pg/mL 范围内的目标 HBeAg 浓度，HBeAg 的检测下限低至 26fg/mL（S/N = 3）。此外，设计的免疫传感器表现出优异的特异性、良好的重现性和可接受的稳定性。在人血清样本分析中的满意结果表明，它在肿瘤标志物的临床监测中有潜在的应用。
