Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China; School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Biosens Bioelectron. 2018 Feb 15;100:512-518. doi: 10.1016/j.bios.2017.09.048. Epub 2017 Sep 28.
An ultrasensitive sandwich-type electrochemical immunosensor was proposed for quantitative detection of hepatitis B surface antigen, which is a representative biomarker of the Hepatitis B virus. First, the porous graphene oxide/Au composites with good conductive ability were employed to accelerate the electron transfer on the electrode interface. Furthermore, the amino functionalized molybdenum disulfide @ cuprous oxide hybrid with coral morphology was prepared to combine platinum nanoparticles for achieving signal amplification strategy. The resulting nanocomposites (molybdenum disulfide @ cuprous oxide - platinum) demonstrated uniform coral morphology, which effectively improved the specific surface area available for loading the secondary antibody and the number of catalytically active sites, even also increased the electrical conductivity. Based on these advantages, this composite system yielded a superior electrocatalytic current response toward the reduction of hydrogen peroxide. In addition, porous graphene oxide/Au composites were used to modify the glassy carbon electrode, thereby presenting a large surface area and becoming biocompatible, for improving the loading capacity of the primary antibody. Under optimal conditions, we obtained a linear relationship between current signal and hepatitis B surface antigen concentration in the broad range from 0.5pg/mL to 200ng/mL, with a detection limit of 0.15pg/mL (signal-to-noise ratio of 3). These values are promising towards clinical applications.
一种超灵敏的三明治型电化学免疫传感器被提出用于乙型肝炎表面抗原的定量检测，它是乙型肝炎病毒的代表性生物标志物。首先，采用具有良好导电能力的多孔氧化石墨烯/金复合材料加速电极界面上的电子转移。此外，还制备了具有珊瑚形态的氨基功能化二硫化钼/氧化亚铜杂化材料，用于结合铂纳米粒子以实现信号放大策略。所得纳米复合材料（二硫化钼/氧化亚铜-铂）呈现均匀的珊瑚形态，有效地增加了用于负载次级抗体的比表面积和催化活性位点的数量，甚至还提高了电导率。基于这些优势，该复合体系在过氧化氢的还原中表现出优异的电催化电流响应。此外，多孔氧化石墨烯/金复合材料被用于修饰玻碳电极，从而呈现出更大的表面积并变得生物相容，以提高初级抗体的负载能力。在最佳条件下，我们在 0.5pg/mL 至 200ng/mL 的宽浓度范围内获得了电流信号与乙型肝炎表面抗原浓度之间的线性关系，检测限为 0.15pg/mL（信噪比为 3）。这些值对于临床应用具有很大的潜力。
