Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
Talanta. 2018 Feb 1;178:481-490. doi: 10.1016/j.talanta.2017.08.034. Epub 2017 Aug 24.
Using sulfonated and fluorinated poly (arylene ether ketone) comprising functional strong coordination group benzimidazole (SPAEK-F-BI) as a template film, a novel fabrication method of cobalt nanoflowers (CoNFs) and non-enzymatic glucose electrochemical sensor was developed in this work. After the precursors Co ions were cooperatively bound by sulfonate and imidazole functionalities contained in SPAEK-F-BI film through ion exchange and strong coordination action, cobalt colloid nuclei were formed and grew to flower-like nanostructures by subsequent in-situ electrochemical reduction on SPAEK-F-BI film modified GCE. Characterization of SPAEK-F-BI film and CoNFs/SPAEK-F-BI film on GCE was performed in detail by FT-IR spectroscopy and scanning electron microscopy (SEM) attached with energy dispersive spectroscopy (EDS), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results of SEM showed that beautiful CoNFs constructed by Co colloid nanosheets with just a few nanometers thickness were well dispersed on uniform SPAEK-F-BI film modified GCE, and the density of CoNFs was mainly influenced by the concentration of the precursor solution CoSO. The CoNFs/SPAEK-F-BI composite modified electrode exhibited good electrocatalytic activity toward glucose oxidation in 0.1M NaOH solution, and the kinetic parameters of glucose oxidation were determined using chronoamperometry. When it was applied for the determination of glucose by amperometry at a potential of 0.6V versus Ag/AgCl, the linear range from 5μM to 1.14mM and the detection limit of 800nM (S/N = 3) were obtained. Finally, it was successfully employed to detect the glucose in human serum real samples, and the results were agreed closely with those measured in hospital.
本工作采用含功能强配位基团苯并咪唑的磺化、氟化聚芳醚酮（SPAEK-F-BI）作为模板膜，开发了一种新型钴纳米花（CoNFs）和非酶葡萄糖电化学传感器的制备方法。前驱体 Co 离子通过离子交换和强配位作用与 SPAEK-F-BI 膜中含有的磺酸基和咪唑基功能团共同配位，形成钴胶体核，随后在 SPAEK-F-BI 膜修饰的 GCE 上通过原位电化学还原，生长成花状纳米结构。通过傅里叶变换红外光谱（FT-IR）和扫描电子显微镜（SEM）以及能量色散光谱（EDS）、电化学阻抗谱（EIS）和循环伏安法（CV）详细表征了 SPAEK-F-BI 膜和 CoNFs/SPAEK-F-BI 膜在 GCE 上的性质。SEM 结果表明，厚度仅有几个纳米的 Co 胶体纳米片组成的美丽 CoNFs 均匀分散在均匀的 SPAEK-F-BI 膜修饰的 GCE 上，CoNFs 的密度主要受前驱体溶液 CoSO 浓度的影响。CoNFs/SPAEK-F-BI 复合修饰电极对 0.1M NaOH 溶液中葡萄糖氧化具有良好的电催化活性，并通过计时电流法测定了葡萄糖氧化的动力学参数。当在 0.6V 对 Ag/AgCl 电位下进行安培法测定葡萄糖时，得到了从 5μM 到 1.14mM 的线性范围和 800nM（S/N = 3）的检测限。最后，它成功地用于检测人血清实际样品中的葡萄糖，并且与医院测量的结果非常吻合。
