Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
Biosens Bioelectron. 2017 May 15;91:380-387. doi: 10.1016/j.bios.2016.12.064. Epub 2016 Dec 30.
The development of efficient and cost effective nonenzymatic biosensors with remarkable sensitivity, selectivity and stability for the detection of biomolecules, especially glucose is one of the major challenges in materials- and electrochemistry. Herein, we report the design and preparation of nonenzymatic biosensor based on an ionic liquid tagged cobalt-salophen metal complex (Co-salophen-IL) immobilized on electrochemically reduced graphene oxide (ERGO) for the detection of glucose via an electrochemical oxidation. The bioinspired Co-salophen-IL complex has been synthesized and immobilized on ERGO, which was previously deposited on a screen printed carbon electrode (SPE) to form the Co-salophen-IL/ERGO/SPE nonenzymatic biosensor. The electrochemical behaviour of this modified electrode was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Notably, the Co-salophen-IL/ERGO/SPE biosensor exhibited excellent electrocatalytic activity towards glucose oxidation in 0.1M NaOH, based on which an amperometric sensor has been developed. The modified electrode has shown prominent performance towards glucose detection over a wide linear range from 0.2µM to 1.8mM with a detection limit and sensitivity of 0.79µM and 62µAmM respectively. The detection was carried out at 0.40V and such a less working potential excludes the interference from the coexisting oxidizable analytes. The role of Co-salophen, IL and ERGO in the electrocatalytic activity has been systematically investigated. Furthermore, the biosensor demonstrated high stability with good reproducibility.
基于离子液体标记钴-席夫碱金属配合物（Co-salophen-IL）固定在电化学还原氧化石墨烯（ERGO）上的非酶生物传感器的设计与制备，用于通过电化学氧化检测生物分子，特别是葡萄糖。生物启发的 Co-salophen-IL 配合物已被合成并固定在 ERGO 上，ERGO 先前沉积在丝网印刷碳电极（SPE）上以形成 Co-salophen-IL/ERGO/SPE 非酶生物传感器。使用循环伏安法（CV）和电化学阻抗谱（EIS）研究了该修饰电极的电化学行为。值得注意的是，Co-salophen-IL/ERGO/SPE 生物传感器在 0.1M NaOH 中对葡萄糖氧化表现出优异的电催化活性，在此基础上开发了安培传感器。该修饰电极在 0.2µM 至 1.8mM 的宽线性范围内对葡萄糖检测表现出突出的性能，检测限和灵敏度分别为 0.79µM 和 62µAmM。检测在 0.40V 进行，如此低的工作电位排除了共存可氧化分析物的干扰。系统研究了 Co-salophen、IL 和 ERGO 在电催化活性中的作用。此外，该生物传感器表现出良好的稳定性和重现性。
