Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
Department of Chemistry, Wuhan University, Wuhan 430072, China.
Talanta. 2018 Feb 1;178:583-587. doi: 10.1016/j.talanta.2017.09.093. Epub 2017 Oct 1.
The demand for fast chromatographic enantioseparation aroused the hot research in stationary phase matrix. In the present study, the flow-through silica, which is characterized by hierarchical pores of through pores in several hundred nanometer range and mesopores about 20nm, was attempted for fast enantioseparation. Thanks to the large surface area and full openness of the through pores, the flow-through silica had comparable cellulose derivative loading amount as the commercial wide-pore silica, which was impracticable for most of the core-shell particles and sub-2-μm fully porous silica. In addition, the backpressure was about two times lower in the case of the flow-through silica of the same particle size to the commercial wide-pore silica, due to the highly porous structure of the former. Another appreciated merit of the flow-through silica in small size (~2µm) was the less dependence of column efficiency on the flow rate. All of the above features rendered the flow-through silica a tremendous potential for fast enantioseparation with desired enantioselectivity, especially suitable for the polysaccharide chiral selector.
对快速色谱手性分离的需求激发了固定相基质的热门研究。在本研究中，尝试了具有数百纳米范围内的贯穿孔和约 20nm 的中孔的直通型硅胶作为快速对映体分离。由于贯穿孔的大表面积和完全开放性，直通型硅胶具有与商业大孔硅胶相当的纤维素衍生物负载量，而商业大孔硅胶对于大多数核壳颗粒和亚 2μm 全多孔硅胶来说是不切实际的。此外，由于前者的高多孔结构，相同粒径的直通型硅胶的背压比商业大孔硅胶低约两倍。直通型硅胶的另一个优点是，在小粒径（约 2μm）时，柱效对流速的依赖性较小。所有这些特性使直通型硅胶在手性选择性方面具有出色的快速对映体分离潜力，特别适用于多糖手性选择剂。
