Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
Department of Chemistry, Materials and Chemical Engineering "G. Natta" Politecnico di Milano, Via Mancinelli 7-20131, Milano, Italy; C.N.R., Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC), Via Mancinelli 7, 20131 Milan, Italy.
J Chromatogr A. 2020 Aug 16;1625:461297. doi: 10.1016/j.chroma.2020.461297. Epub 2020 Jun 11.
In this study superficially porous silica particles with a nominal pore size of 450 Å and average particle size of 2.6 micrometers was compared to fully porous silica particles with nominal particle size 3 micrometers and nominal pore size 1000 A as carriers for a polysaccharide based chiral selector for the separation of enantiomers in high-performance liquid chromatography. In addition, the effects of chiral selector loading onto the silica support and of column internal dimeter in the case of both, superficially porous and totally porous silica, as well as of the pore size of superficially porous silica on column performance were studied. The dependence of plate height on mobile phase flow rate was also studied and attempts were made for shortening analysis time. The baseline separation of enantiomers of some chiral sulfoxides was obtained within 2.0-4.5 s.
在这项研究中，将标称孔径为 450Å、平均粒径为 2.6 微米的表面多孔硅胶颗粒与标称粒径为 3 微米、标称孔径为 1000Å 的全多孔硅胶颗粒进行了比较，它们均作为多糖类手性选择剂的载体，用于高效液相色谱中对映异构体的分离。此外，还研究了手性选择剂在硅胶载体上的负载量以及在表面多孔硅胶和全多孔硅胶的情况下柱内径的影响，以及表面多孔硅胶的孔径对柱性能的影响。还研究了板高随流动相流速的变化，并尝试缩短分析时间。一些手性亚砜对映异构体在 2.0-4.5s 内实现了基线分离。
