Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia; Faculty of Natural Sciences and Healthcare, Sokhumi State University, Polytkovskaya 9, 0186 Tbilisi, Georgia.
J Chromatogr A. 2018 Oct 12;1571:132-139. doi: 10.1016/j.chroma.2018.08.011. Epub 2018 Aug 4.
Our earlier studies have demonstrated the applicability of polysaccharide-based chiral selectors in combination with superficially porous (or core-shell) silica (SPS) particles for the preparation of highly efficient chiral stationary phases (CSP). In earlier studies, CSPs were prepared by coating (adsorption) of the chiral selector onto the surface of silica. In this study we report for the first time the CSP obtained by covalent immobilization of a chiral selector onto the surface of SPS particles. The applicability of this CSP for the separation of enantiomers in pure methanol and acetonitrile, as well as in n-hexane/2-propanol mobile phases is shown. The effect of the injected sample amount, mobile phase flow rate and detection frequency on separation performance were studied, as well as high efficiency separation of enantiomers with the analysis time less than 30 s was attempted.
我们之前的研究已经证明，多糖基手性选择剂与表面多孔（或核壳）硅胶（SPS）颗粒结合用于制备高效手性固定相（CSP）的适用性。在早期的研究中，CSP 是通过将手性选择剂涂覆（吸附）在硅胶表面上制备的。在这项研究中，我们首次报道了通过将手性选择剂共价固定在 SPS 颗粒表面上获得的 CSP。展示了该 CSP 在纯甲醇和乙腈以及正己烷/2-丙醇流动相中分离对映异构体的适用性。研究了注入样品量、流动相流速和检测频率对分离性能的影响，并且尝试了用少于 30 秒的分析时间实现高效分离对映异构体。
