Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ-500 01 Hradec Kralove, Czech Republic.
Talanta. 2018 Feb 1;178:970-973. doi: 10.1016/j.talanta.2017.10.031. Epub 2017 Oct 24.
Tumor Necrosis Factor Alpha (TNFα) is an important marker of inflammatory processes in human body. In the current healthcare, determination of TNFα blood or plasma level is done by Enzyme Linked Immuno-Sorbent Assay (ELISA) as a primary choice method. Piezoelectric immunosensors are analytical platform recording affinity interactions on their surface. It is inferred that the immunosensors would be a functional alternative to the ELISA. In this study, antibody against TNFα was immobilized on Quartz Crystal Microbalance (QCM) sensor and the same was made on magnetic particles. Human TNFα was measured in a way of interaction with QCM surface and then the particles were applied. The assay exerted sufficient limit of detection equal to 1.62pg/ml and it also fully correlated with standard ELISA tests. No interference by interleukin 6 or albumin was observed. Long term stability of the immunosensors lasting for at least three months was found. The immunosensors appears to be readily for practical performance and it would be an alternative to the standard ELISA especially when diagnoses made in field, homecare conditions or conditions of small hospitals as an emergency test.
肿瘤坏死因子-α（TNFα）是人体炎症过程的一个重要标志物。在当前的医疗保健中，通过酶联免疫吸附测定（ELISA）作为首选方法来测定 TNFα 的血液或血浆水平。压电免疫传感器是一种记录其表面亲和力相互作用的分析平台。据推断，免疫传感器将是 ELISA 的一种功能替代方法。在这项研究中，针对 TNFα 的抗体被固定在石英晶体微天平（QCM）传感器上，并用磁性粒子进行了同样的处理。通过与 QCM 表面相互作用的方式来测量人 TNFα，然后应用这些粒子。该测定法的检测限足够低，为 1.62pg/ml，并且与标准 ELISA 测试完全相关。未观察到白细胞介素 6 或白蛋白的干扰。发现免疫传感器具有至少三个月的长期稳定性。免疫传感器似乎易于实际应用，并且将成为标准 ELISA 的替代方法，特别是在现场、家庭护理或小型医院的紧急情况下进行诊断时。
