Pohanka Miroslav
Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove, CZ-50001, Czech Republic.
Talanta. 2020 Oct 1;218:121167. doi: 10.1016/j.talanta.2020.121167. Epub 2020 May 21.
Interferon gamma (IFNγ) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFNγ are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor working on the principle of quartz crystal microbalance (QCM) was developed as an alternative to the standard analytical methods for IFNγ. The biosensor contained antibodies against IFNγ immobilized on QCM and also on gold nanoparticles. A sandwich containing QCM, gold nanoparticles and IFNγ was formed and formation of the sandwich caused decrease of oscillation frequency. The assay exerted limit of detection 5.7 pg/ml for a sample sized 50 μl and one measuring cycle was finished within 90 min. The assay by biosensor fully correlated to standard ELISA. In a conclusion, the biosensor appears to be a fully applicable analytical tool for a simple assay of IFNγ. Overall simplicity and no special requirement on staff and equipment are the major advantages of the here presented assay.
γ干扰素（IFNγ）是一种细胞因子和免疫化学标志物，可用于揭示传染病，特别是用于区分病毒感染和某些类型的细菌感染。检测IFNγ的血液检测通常基于免疫测定法，如酶联免疫吸附测定（ELISA）。在本文中，开发了一种基于石英晶体微天平（QCM）原理的生物传感器，作为IFNγ标准分析方法的替代方法。该生物传感器包含固定在QCM以及金纳米颗粒上的抗IFNγ抗体。形成了一个包含QCM、金纳米颗粒和IFNγ的夹心结构，夹心结构的形成导致振荡频率降低。对于50μl的样品，该检测方法的检测限为5.7 pg/ml，一个测量周期在90分钟内完成。该生物传感器检测方法与标准ELISA完全相关。总之，该生物传感器似乎是一种完全适用的分析工具，可用于简单检测IFNγ。整体简单性以及对人员和设备无特殊要求是本文所述检测方法的主要优点。
