Ginders Maximilian, Leschnik Michael, Künzel Frank, Kampner Doris, Mikula Claudia, Steindl Georg, Eichhorn Inga, Feßler Andrea T, Schwarz Stefan, Spergser Joachim, Loncaric Igor
Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria.
Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria.
Acta Vet Scand. 2017 Nov 14;59(1):79. doi: 10.1186/s13028-017-0348-2.
The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017.
Isolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype.
This study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.
本研究的目的是调查从奥地利伴侣动物和马匹中分离出的肺炎链球菌菌株的遗传相关性和抗菌药物耐药性谱。在2009年3月至2017年1月的常规诊断活动中，共获得了12株经初步鉴定为肺炎链球菌的非重复菌株。
通过胆汁溶解试验、奥普托欣敏感性试验、基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析以及recA部分和16S rRNA基因的序列分析，确认分离株为肺炎链球菌。通过肺炎溶血素聚合酶链反应（PCR）对分离株进行进一步鉴定，并通过多位点序列分型（MLST）进行基因分型。进行了抗菌药物敏感性试验，并通过特异性PCR检测耐药基因。对所有分离株进行了血清分型。检测到四种序列类型（ST）（ST36、ST3546、ST6934和ST6937）和四种血清型（3、19A、19F和23F）。12株分离株中有2株表现出多重耐药表型和基因型。
本研究是对从奥地利伴侣动物和马匹中分离出的肺炎链球菌菌株特征的首次全面调查。所得结果表明，与侵袭性肺炎球菌疾病（IPD）相关的常见人类血清型（23F）和序列类型（ST36）可能在犬类中传播。从其他受试动物中获得的分离株似乎具有宿主适应性。
