College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Environ Pollut. 2018 Jun;237:775-783. doi: 10.1016/j.envpol.2017.10.129. Epub 2017 Nov 11.
Propamocarb (PM) is a widely used fungicide with property of affecting fatty acid and phospholipid biosynthesis in funguses. In this study, we explored its effects on mice gut microbiota and metabolism by exposing mice to 3, 30, and 300 mg/L PM through drinking water for a duration of 28 days. We observed that the transcription of hepatic genes related to regulate lipid metabolism were perturbed by PM exposure. The microbiota in the cecal contents and feces changed during or after PM exposure at phylum or genus levels. 16S rRNA gene sequencing for the cecal content revealed shifted in overall microbial structure after PM exposure, and operational taxonomic unit (OTU) analysis indicated that 32.2% of OTUs changed by 300 mg/mL PM exposure for 28 days. In addition, based on H NMR analysis,a total of 20 fecal metabolites mainly including succinate, short chain fatty acids, bile acids and trimethylamine were found to be significantly influenced by exposure to 300 mg/L PM.,. These metabolites were tightly correlated to host metabolism. Our findings indicated that high doses of PM exposure could disturb mice metabolism through, or partly through, altering the gut microbiota and microbial metabolites.
丙环唑（PM）是一种广泛使用的杀菌剂，具有影响真菌中脂肪酸和磷脂生物合成的特性。在这项研究中，我们通过饮用水将小鼠暴露于 3、30 和 300mg/L PM 中 28 天，来研究其对小鼠肠道微生物群和代谢的影响。我们观察到 PM 暴露会干扰肝脏中与脂质代谢调节相关的基因的转录。在 PM 暴露期间或之后，盲肠内容物和粪便中的微生物群在门或属水平上发生变化。16S rRNA 基因测序揭示了 PM 暴露后整体微生物结构的变化，操作分类单元（OTU）分析表明，300mg/mL PM 暴露 28 天可使 32.2%的 OTU 发生变化。此外，基于 H NMR 分析，共发现 20 种粪便代谢物主要包括琥珀酸、短链脂肪酸、胆汁酸和三甲胺，它们受 300mg/L PM 暴露的影响显著。这些代谢物与宿主代谢密切相关。我们的研究结果表明，高剂量的 PM 暴露可能通过改变肠道微生物群和微生物代谢物来干扰小鼠的代谢。
