College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Environment science, Zhejiang University of Technology, Hangzhou 310032, China.
Sci Total Environ. 2018 Aug 1;631-632:439-448. doi: 10.1016/j.scitotenv.2018.03.053. Epub 2018 Mar 16.
Lead (Pb) is one of the most prevalent toxic, nonessential heavy metals that can contaminate food and water. In this study, effects of chronic exposure to low concentrations of Pb on metabolism and gut microbiota were evaluated in mice. It was observed that exposure of mice to 0.1mg/L Pb, supplied via drinking water, for 15weeks increased hepatic TG and TCH levels. The levels of some key genes related to lipid metabolism in the liver increased significantly in Pb-treated mice. For the gut microbiota, at the phylum level, the relative abundance of Firmicutes and Bacteroidetes changed obviously in the feces and the cecal contents of mice exposed to 0.1mg/L Pb for 15weeks. In addition, 16s rRNA gene sequencing further discovered that Pb exposure affected the structure and richness of the gut microbiota. Moreover, a H NMR metabolic analysis unambiguously identified 31 metabolites, and 15 metabolites were noticeably altered in 0.1mg/L Pb-treated mice. Taken together, the data indicate that chronic Pb exposure induces dysbiosis of the gut microbiota and metabolic disorder in mice.
Chronic Pb exposure induces metabolic disorder, dysbiosis of the gut microbiota and hepatic lipid metabolism disorder in mice.
铅（Pb）是最常见的有毒、非必需重金属之一，可污染食物和水。在这项研究中，评估了慢性暴露于低浓度 Pb 对小鼠代谢和肠道微生物群的影响。结果表明，通过饮用水向小鼠提供 0.1mg/L Pb，暴露 15 周会增加肝脏 TG 和 TCH 水平。Pb 处理小鼠肝脏中一些与脂质代谢相关的关键基因水平显著升高。对于肠道微生物群，在门水平上，暴露于 0.1mg/L Pb 15 周的小鼠粪便和盲肠内容物中厚壁菌门和拟杆菌门的相对丰度明显改变。此外，16s rRNA 基因测序进一步发现 Pb 暴露影响了肠道微生物群的结构和丰富度。此外，NMR 代谢分析明确鉴定出 31 种代谢物，其中 15 种代谢物在 0.1mg/L Pb 处理的小鼠中明显改变。总之，这些数据表明，慢性 Pb 暴露可诱导小鼠肠道微生物群失调和代谢紊乱以及肝脂质代谢紊乱。
