杂交DNA i-基序：氨乙基脯氨酰肽核酸（pC）增强了DNA（dC）i-基序结构的稳定性。

Hybrid DNA i-motif: Aminoethylprolyl-PNA (pC) enhance the stability of DNA (dC) i-motif structure.

作者信息

Gade Chandrasekhar Reddy, Sharma Nagendra K

机构信息

School of Chemical Sciences, National Institute of Science Education and Research (NISER), HBNI-Mumbai, Bhubaneswar, Jatni 752050, Odisha, India.

出版信息

Bioorg Med Chem Lett. 2017 Dec 15;27(24):5424-5428. doi: 10.1016/j.bmcl.2017.11.004. Epub 2017 Nov 3.

DOI:10.1016/j.bmcl.2017.11.004

PMID:29138026

Abstract

This report describes the synthesis of C-rich sequence, cytosine pentamer, of aep-PNA and its biophysical studies for the formation of hybrid DNA:aep-PNAi-motif structure with DNA cytosine pentamer (dC) under acidic pH conditions. Herein, the CD/UV/NMR/ESI-Mass studies strongly support the formation of stable hybrid DNA i-motif structure with aep-PNA even near acidic conditions. Hence aep-PNA C-rich sequence cytosine could be considered as potential DNA i-motif stabilizing agents in vivo conditions.

摘要

本报告描述了富含C序列的aep - PNA胞嘧啶五聚体的合成及其在酸性pH条件下与DNA胞嘧啶五聚体（dC）形成杂交DNA：aep - PNA i - 基序结构的生物物理研究。在此，圆二色光谱/紫外光谱/核磁共振/电喷雾质谱研究有力地支持了即使在接近酸性条件下aep - PNA也能形成稳定的杂交DNA i - 基序结构。因此，富含aep - PNA C序列的胞嘧啶可被视为体内条件下潜在的DNA i - 基序稳定剂。