Department of Culinary Art, I-Shou University, Kaohsiung, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Sci Rep. 2017 Nov 14;7(1):15515. doi: 10.1038/s41598-017-15627-8.
Bacterial contamination of ready-to-eat (RTE) street foods is a major concern worldwide. Dissemination of antibiotic resistant pathogens from food is an emerging public-health threat. To investigate the prevalence of antibiotic resistance genes and ceftazidime resistance-associated efflux pumps in foodborne pathogens, 270 RTE street foods samples were collected in three densely populated Taiwanese cities. Among 70 ceftazidime non-susceptible isolates, 21 Stenotrophomonas maltophilia, 12 Pseudomonas spp., 22 Acinetobacter spp., and 15 Enterobacteriaceae isolates were identified. Phylogenetic analyses revealed high levels of genetic diversity between all of the different strains. Multi-drug resistance was observed in 86.4% (19/22) of Acinetobacter spp., 100% (12/12) of Pseudomonas spp., 71.4% (15/21) of S. maltophilia, and 93.3% (14/15) of Enterobacteriaceae. Of 70 ceftazidime non-susceptible isolates, 13 contained ESBLs or plasmid-mediated ampC genes and 23 contained ceftazidime resistance-associated efflux pumps, with Acinetobacter spp. identified as predominant isolate (69.6%; 16/23). AdeIJK pump RNA expression in Acinetobacter isolates was 1.9- to 2-fold higher in active efflux strains. Nine clinically resistant genes were detected: catIII and cmlA (chloramphenicol); aacC1, aacC2, aacC3, and aacC4 (gentamicin); tet(A), tet(C), and tet(D) (tetracycline). The scope and abundance of multidrug-resistant bacteria described in this report underscores the need for ongoing and/or expanded RTE monitoring and control measures.
食源性致病菌中抗生素耐药基因与头孢他啶耐药相关外排泵的流行状况
即食街头食品（RTE）的细菌污染是全球范围内的一个主要关注点。食源性病原体传播抗生素耐药性是一个新出现的公共卫生威胁。为了调查食源性致病菌中抗生素耐药基因和头孢他啶耐药相关外排泵的流行情况，在台湾三个人口密集的城市中采集了 270 份即食街头食品样本。在 70 株头孢他啶非敏感性分离株中，鉴定出 21 株嗜麦芽窄食单胞菌、12 株假单胞菌、22 株不动杆菌和 15 株肠杆菌科分离株。系统发育分析显示，所有不同菌株之间存在高度的遗传多样性。22 株不动杆菌中 86.4%（19/22）、12 株假单胞菌中 100%（12/12）、21 株嗜麦芽窄食单胞菌中 71.4%（15/21）和 15 株肠杆菌科中 93.3%（14/15）表现出多药耐药性。在 70 株头孢他啶非敏感性分离株中，13 株含有 ESBLs 或质粒介导的 AmpC 基因，23 株含有头孢他啶耐药相关外排泵，其中不动杆菌是主要分离株（69.6%；16/23）。在活性外排株中，AdeIJK 泵 RNA 表达增加 1.9-2 倍。检测到 9 种临床耐药基因：catIII 和 cmlA（氯霉素）；aacC1、aacC2、aacC3 和 aacC4（庆大霉素）；tet(A)、tet(C)和 tet(D)（四环素）。本报告描述的多药耐药菌的范围和丰度强调了需要持续进行和/或扩大即食食品监测和控制措施。
