Matjašič Alenka, Tajnik Mojca, Boštjančič Emanuela, Popović Mara, Matos Boštjan, Glavač Damjan
Department of Molecular Genetics, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Int J Genomics. 2017;2017:2312318. doi: 10.1155/2017/2312318. Epub 2017 Sep 12.
Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play a significant role in cancer development as regulators of protein-coding genes. Their dysregulation was in some extent already associated with glioma, the most aggressive primary brain tumours in adults. The correct diagnosis and treatment selection due to high tumour heterogeneity might be difficult and inadequate, resulting in poor prognosis. Studies of expression patterns of noncoding RNAs (ncRNAs) could provide useful insight in glioma molecular development. We used the qPCR approach to screen and investigate the expression of lncRNAs that were previously deregulated in other cancer types. The study showed altered expression levels for numerous lncRNAs across histologically different glioma samples. Validation of few lncRNAs showed association of expression levels with histological subtype and/or malignancy grade. We also observed deregulated and subtype-distinctive expression for four lncRNA-associated miRNAs. Expression of few lncRNAs and miRNA was also associated with patients' survival, showing potential prognostic value. Several ncRNAs, some already related to glioma and some, to the best of our knowledge, investigated for the first time, might be of greater importance in glioma molecular development and progression. Finding the subtype-specific lncRNA and/or miRNA expression patterns may contribute additional information for a more objective classification.
长链非编码RNA（lncRNAs）和微小RNA（miRNAs）作为蛋白质编码基因的调节因子，在癌症发展中发挥着重要作用。它们的失调在一定程度上已与胶质瘤相关，胶质瘤是成人中最具侵袭性的原发性脑肿瘤。由于肿瘤高度异质性，正确的诊断和治疗选择可能困难且不充分，导致预后不良。对非编码RNA（ncRNAs）表达模式的研究可为胶质瘤的分子发展提供有用的见解。我们使用定量聚合酶链反应（qPCR）方法筛选并研究了先前在其他癌症类型中失调的lncRNAs的表达。该研究表明，在组织学不同的胶质瘤样本中，许多lncRNAs的表达水平发生了改变。对少数lncRNAs的验证表明，其表达水平与组织学亚型和/或恶性程度相关。我们还观察到四种lncRNA相关miRNAs的失调和亚型特异性表达。少数lncRNAs和miRNA的表达也与患者的生存相关，显示出潜在的预后价值。一些ncRNAs，有些已经与胶质瘤相关，有些据我们所知是首次研究，可能在胶质瘤的分子发展和进展中具有更重要的意义。找到亚型特异性的lncRNA和/或miRNA表达模式可能会为更客观的分类提供额外信息。
