School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi'an, Shaanxi 710061, P.R. China.
Mol Med Rep. 2018 Jan;17(1):1833-1838. doi: 10.3892/mmr.2017.8057. Epub 2017 Nov 14.
Numerous studies have focused on osteoarthritis (OA) cartilage injuries, with observations of abnormalities in microRNA (miRNA) expression levels. The aim of the present study was to consolidate and assess the results of these studies to identify potential miRNA biomarkers of OA. A comprehensive meta‑analysis of eight independent miRNA expression studies in OA was performed using the robust rank aggregation method (RRA), which contained a total of 82 OA and 39 normal cartilage samples. The targets of meta‑signature miRNA were predicted using TargetScan v6.2, PicTar, miRDB, TarBase v7.0 and starBase databases. In addition, pathway enrichment analysis was performed to establish the biologically and functionally relevant genes involved in meta‑signature miRNA regulation. Six dysregulated miRNAs were identified, including four up regulated (miR‑23b‑3p, miR‑27b‑3p, miR‑211‑5p and miR‑16‑5p) and two down regulated (miR‑25‑3p and miR‑149‑5p). The enrichment of Kyoto Encyclopedia of Genes and Genomes and Panther signaling pathways were predominantly associated with cell signaling and cell regulation. In the present study, the meta‑signature miRNA identified may be used to develop a series of diagnostic and prognostic biomarkers for OA specifically for use in a clinical setting.
许多研究都集中在骨关节炎（OA）软骨损伤上，观察到 microRNA（miRNA）表达水平异常。本研究旨在整合和评估这些研究的结果，以确定 OA 的潜在 miRNA 生物标志物。使用稳健等级聚合方法（RRA）对 8 项 OA 独立 miRNA 表达研究进行了综合荟萃分析，其中包含 82 个 OA 和 39 个正常软骨样本。使用 TargetScan v6.2、PicTar、miRDB、TarBase v7.0 和 starBase 数据库预测了元特征 miRNA 的靶标。此外，还进行了通路富集分析，以确定参与元特征 miRNA 调控的生物学和功能相关基因。鉴定出 6 个失调 miRNA，包括 4 个上调 miRNA（miR-23b-3p、miR-27b-3p、miR-211-5p 和 miR-16-5p）和 2 个下调 miRNA（miR-25-3p 和 miR-149-5p）。京都基因与基因组百科全书和 Panther 信号通路的富集主要与细胞信号和细胞调节有关。在本研究中，所确定的元特征 miRNA 可用于开发一系列针对 OA 的诊断和预后生物标志物，特别适用于临床应用。
