Department of Cell and Molecular Biology, Uppsala University, BMC Box 596, S-751 24 Uppsala, Sweden.
Departamento de Quimica Fisica, Facultad de Ciencias, University of Granada, 18071 Granada, Spain.
Curr Opin Struct Biol. 2018 Feb;48:83-92. doi: 10.1016/j.sbi.2017.10.020. Epub 2017 Nov 12.
Enzymes are flexible catalysts, and there has been substantial discussion about the extent to which this flexibility contributes to their catalytic efficiency. What has been significantly less discussed is the extent to which this flexibility contributes to their evolvability. Despite this, recent years have seen an increasing number of both experimental and computational studies that demonstrate that cooperativity and flexibility play significant roles in enzyme innovation. This review covers key developments in the field that emphasize the importance of enzyme dynamics not just to the evolution of new enzyme function(s), but also as a property that can be harnessed in the design of new artificial enzymes.
酶是灵活的催化剂，人们已经对这种灵活性在多大程度上有助于提高其催化效率进行了大量的讨论。但人们讨论得较少的是，这种灵活性在多大程度上有助于提高其可进化性。尽管如此，近年来越来越多的实验和计算研究表明，协同作用和灵活性在酶的创新中起着重要作用。本综述涵盖了该领域的关键发展，强调了酶动力学的重要性，不仅对新酶功能的进化很重要，而且还可以作为一种可以在新的人工酶设计中利用的特性。
