Hassanpour Seyed Esmail, Tarahomi Mohammadreza, Daryani Amir
Department of Plastic Surgery, Shahid-Beheshti Medical University, 15 Khordad Hospital, Tehran, the Islamic Republic of Iran.
J Reconstr Microsurg. 2018 Mar;34(3):206-210. doi: 10.1055/s-0037-1607437. Epub 2017 Nov 15.
Revascularized digits may fail because of vessel thrombosis. The current study aimed to develop a standard model for crush injury in the rat femoral artery to evaluate the antithrombotic effect of perivascular botulinum toxin A (BTX-A) injection.
We explored bilateral femoral arteries of 10 rats using a microscopic technique. The crush injury was reproduced using a bulldog clamp. One randomly chosen leg of each rat was injected with BTX-A. The other side received normal saline as its own control. After 24 hours, the femoral arteries were ligated distal to the trauma site and were divided between the traumatized and ligated sites.
All arteries injected with BTX-A had pulsatile bleeding without thrombosis. Arteries injected with normal saline had thrombosis without bleeding, except for one with oozing. The results showed the efficacy of BTX-A and its potential use in microvascular trauma ( < 0.001).
The use of BTX-A preoperatively in the emergency department may help reduce traumatic stress and subsequent thrombosis and improve trauma management results. Complementary studies are needed to assess the ability of BTX-A to reduce thrombosis and vasospasm and improve survival of traumatized digits.
再植指可能因血管血栓形成而失败。本研究旨在建立大鼠股动脉挤压伤的标准模型，以评估血管周围注射肉毒杆菌毒素A（BTX-A）的抗血栓形成作用。
我们采用显微技术对10只大鼠的双侧股动脉进行探查。使用动脉夹重现挤压伤。每只大鼠随机选择一条腿注射BTX-A。另一侧注射生理盐水作为自身对照。24小时后，在创伤部位远端结扎股动脉，并在创伤和结扎部位之间进行分离。
所有注射BTX-A的动脉均有搏动性出血且无血栓形成。注射生理盐水的动脉除一例外均有血栓形成且无出血，该例有渗血。结果显示了BTX-A的有效性及其在微血管创伤中的潜在应用价值（<0.001）。
在急诊科术前使用BTX-A可能有助于减轻创伤应激及随后的血栓形成，并改善创伤处理结果。需要进行补充研究以评估BTX-A减少血栓形成和血管痉挛以及提高创伤指存活的能力。
