MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Biosens Bioelectron. 2016 Aug 15;82:127-31. doi: 10.1016/j.bios.2016.03.067. Epub 2016 Mar 26.
In this paper, a magnetoelastic (ME) sensing system for the detection of classical swine fever virus (CSFV) is presented. The detection system comprises a test paper and a measurement circuit. The test paper consists mainly of an ME biosensor to detect the CSFV. Based on the impedance analysis technique, the measurement circuit is designed to measure the resonance frequency of the ME biosensor. The anti-CSFV IgG is immobilized onto the ME sensor surface to form the ME biosensor through a physical absorption approach. The experimental results show that the shift in the resonance frequency of the ME biosensor increases with the augmentation of the CSFV concentration. The effectiveness of the combination between the anti-CSFV IgG and CSFV is confirmed by the scanning electron microscopy (SEM) images, the sandwich-based enzyme-linked immunosorbent assay (ELISA) analysis, the interference study and the reference biosensor test method. The resonance frequency shift is linearly proportional to the concentration in the range from 0 to 2.5μg/ml, and becomes sub-linear at higher concentrations. The ME biosensor for CSFV detection has a sensitivity of about 95Hz/μg·ml(-1), with a detection limit of 0.6μg/ml.
本文提出了一种用于检测经典猪瘟病毒（CSFV）的磁弹性（ME）传感系统。该检测系统包括试纸和测量电路。试纸主要由用于检测 CSFV 的 ME 生物传感器组成。基于阻抗分析技术，设计了测量电路来测量 ME 生物传感器的共振频率。通过物理吸收方法，将抗 CSFV IgG 固定在 ME 传感器表面上以形成 ME 生物传感器。实验结果表明，ME 生物传感器的共振频率位移随着 CSFV 浓度的增加而增加。通过扫描电子显微镜（SEM）图像、基于三明治的酶联免疫吸附测定（ELISA）分析、干扰研究和参考生物传感器测试方法证实了抗 CSFV IgG 和 CSFV 之间的结合的有效性。在 0 至 2.5μg/ml 的范围内，共振频率位移与浓度呈线性关系，而在较高浓度下呈亚线性关系。用于 CSFV 检测的 ME 生物传感器的灵敏度约为 95Hz/μg·ml(-1)，检测限为 0.6μg/ml。
