de Aretxabala X, Yonemura Y, Sugiyama K, Hirose N, Kumaki T, Fushida S, Miwa K, Miyazaki I
Department of Surgery II, School of Medicine, Kanazawa University, Japan.
Cancer. 1989 Feb 15;63(4):791-8. doi: 10.1002/1097-0142(19890215)63:4<791::aid-cncr2820630431>3.0.co;2-w.
This study was carried out on 222 samples from 37 gastric carcinomas to assess the incidence of multiple stem lines in primary tumors and metastasis as reflected by multiple DNA stem lines and their relationship to epidermal growth factor (EGF) receptor expression, histologic grade, tumor size, and degree of wall infiltration. Fifteen primary tumors (40.5%) were homogeneously diploid/peridiploid whereas 22 (59.5%) were aneuploid. In the lymph node metastasis, seven patients (29.2%) had an homogeneous diploid/peridiploid pattern in all metastatic lymph nodes. On the other hand, 17 (70.8%) had at least one aneuploid peak in the lymph node metastasis. DNA content heterogeneity was seen in 12 (33%) of primary tumors whereas 14 (66.6%) of 21 patients had multiple cell clones in the metastasis. Therefore, 12 patients had a metastatic clone which was not observed in the primary tumor. DNA content heterogeneity was seen even in tumors with submucosal invasion suggesting that this phenomenon is also present at earlier stages. No correlation between the histologic grade and the DNA distribution was observed. Furthermore, histologic heterogeneity was independent of DNA content heterogeneity. The EGF receptor expression was observed in six of the 23 patients in whom this analysis was done. The EGF receptor expression was constant in all samples which were studied and even samples with a different DNA content and histologic grade were stables for the EGF receptor expression.
本研究对来自37例胃癌的222个样本进行，以评估原发性肿瘤和转移灶中多干细胞系的发生率，这可通过多个DNA干细胞系反映出来，以及它们与表皮生长因子（EGF）受体表达、组织学分级、肿瘤大小和壁浸润程度的关系。15个原发性肿瘤（40.5%）为均匀二倍体/亚二倍体，而22个（59.5%）为非整倍体。在淋巴结转移中，7例患者（29.2%）所有转移淋巴结均为均匀二倍体/亚二倍体模式。另一方面，17例（70.8%）在淋巴结转移中至少有一个非整倍体峰。12个原发性肿瘤（33%）可见DNA含量异质性，而21例患者中有14例（66.6%）在转移灶中有多个细胞克隆。因此，12例患者的转移克隆在原发性肿瘤中未观察到。即使在有黏膜下浸润的肿瘤中也可见DNA含量异质性，提示这种现象在早期阶段也存在。未观察到组织学分级与DNA分布之间的相关性。此外，组织学异质性与DNA含量异质性无关。在进行此项分析的23例患者中，有6例观察到EGF受体表达。在所有研究样本中均观察到EGF受体表达恒定，即使是DNA含量和组织学分级不同的样本，其EGF受体表达也是稳定的。
