Ooiwa H, Saito A, Tomisaki S, Oshiro T, Okuyama T, Oono S, Baba H, Maehara Y, Sugimachi K
Dept. of Surgery II, Faculty of Medicine, Kyushu University.
Gan To Kagaku Ryoho. 1994 May;21 Suppl 1:17-20.
DNA ploidy was microspectrophotometrically investigated in 46 patients with gastric carcinoma. Measurements of DNA content and mitotic index (MI) were examined in the mucosal, submucosal, muscularis propria, and serosal layers of tumors, respectively. The frequency of cells with values exceeding hexaploid chromosome (6c) and mitotic counting analysis revealed a significantly higher value in the serosa than in the mucosa. This tendency was not evident in differentiated type adenocarcinoma but was noted in those with the undifferentiated type. There were 37 tumors (80.0%) with the same DNA distribution pattern in every layer of the stomach (homogeneous DNA ploidy). Heterogeneity of DNA ploidy was observed in nine tumors (20.0%). Carcinoma with heterogeneous DNA ploidy manifested a significantly higher incidence of metastasis to lymph nodes than did those with the homogeneous type. Characteristically, there was venous permeation preponderance in the differentiated type and peritoneal dissemination preponderance in the undifferentiated type. This evidence of DNA heterogeneity in gastric carcinoma tissue suggests a possible correlation with metastatic behavior.
对46例胃癌患者的DNA倍体进行了显微分光光度法研究。分别检测了肿瘤黏膜层、黏膜下层、固有肌层和浆膜层的DNA含量和有丝分裂指数（MI）。DNA值超过六倍体染色体（6c）的细胞频率和有丝分裂计数分析显示，浆膜层的值显著高于黏膜层。这种趋势在分化型腺癌中不明显，但在未分化型腺癌中较为明显。37个肿瘤（80.0%）在胃的各层中具有相同的DNA分布模式（DNA倍体均匀）。9个肿瘤（20.0%）观察到DNA倍体异质性。DNA倍体异质性的癌转移至淋巴结的发生率显著高于DNA倍体均匀型。特征性地，分化型以静脉浸润为主，未分化型以腹膜播散为主。胃癌组织中这种DNA异质性的证据表明其可能与转移行为相关。
