聚合酶链反应检测饮用水源中大肠菌群的评估
Evaluation of Polymerase Chain Reaction for Detecting Coliform Bacteria in Drinking Water Sources.
作者信息
Isfahani Bahram Nasr, Fazeli Hossein, Babaie Zeinab, Poursina Farkhondeh, Moghim Sharareh, Rouzbahani Meisam
机构信息
Department of Microbiology and Virology, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Adv Biomed Res. 2017 Oct 16;6:130. doi: 10.4103/2277-9175.216783. eCollection 2017.
BACKGROUND
Coliform bacteria are used as indicator organisms for detecting fecal pollution in water. Traditional methods including microbial culture tests in lactose-containing media and enzyme-based tests for the detection of β-galactosidase; however, these methods are time-consuming and less specific. The aim of this study was to evaluate polymerase chain reaction (PCR) for detecting coliform.
MATERIALS AND METHODS
Totally, 100 of water samples from Isfahan drinking water source were collected. Coliform bacteria and were detected in drinking water using LacZ and LamB genes in PCR method performed in comparison with biochemical tests for all samples.
RESULTS
Using phenotyping, 80 coliform isolates were found. The results of the biochemical tests illustrated 78.7% coliform bacteria and 21.2% . PCR results for LacZ and LamB genes were 67.5% and 17.5%, respectively.
CONCLUSION
The PCR method was shown to be an effective, sensitive, and rapid method for detecting coliform and in drinking water from the Isfahan drinking water sources.
背景
大肠菌群被用作检测水中粪便污染的指示生物。传统方法包括在含乳糖培养基中进行微生物培养试验以及基于酶的β-半乳糖苷酶检测试验；然而，这些方法耗时且特异性较低。本研究的目的是评估聚合酶链反应（PCR）用于检测大肠菌群。
材料与方法
总共收集了100份来自伊斯法罕饮用水源的水样。使用PCR方法中的LacZ和LamB基因检测饮用水中的大肠菌群，并与所有样品的生化试验进行比较。
结果
通过表型分析，发现了80株大肠菌群分离株。生化试验结果显示大肠菌群为78.7%，其他菌为21.2%。LacZ和LamB基因的PCR结果分别为67.5%和17.5%。
结论
PCR方法被证明是一种检测伊斯法罕饮用水源饮用水中大肠菌群和其他菌的有效、灵敏且快速的方法。
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