School of Materials Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Nanoscale. 2017 Dec 21;10(1):158-166. doi: 10.1039/c7nr07367d.
Synthesis of new, highly active antibacterial agents has become increasingly important in light of emerging antibiotic resistance. In the present study, ZnO/graphene quantum dot (GQD) nanocomposites were produced by a facile hydrothermal method and characterized by an array of microscopic and spectroscopic measurements, including transmission electron microscopy, X-ray photoelectron spectroscopy, UV-vis and photoluminescence spectroscopy. Antibacterial activity of the ZnO/GQD nanocomposites was evaluated with Escherichia coli within the context of minimum inhibitory concentration and the reduction of the number of bacterial colonies in a standard plate count method, in comparison to those with ZnO and GQD separately. It was found that the activity was markedly enhanced under UV photoirradiation as compared to that in ambient light. This was ascribed to the enhanced generation of reactive oxygen species under UV photoirradiation, with minor contributions from membrane damage, as manifested in electron paramagnetic resonance and fluorescence microscopic measurements. The results highlight the significance of functional nanocomposites based on semiconductor nanoparticles and graphene derivatives in the development of effective bactericidal agents.
鉴于抗生素耐药性的出现，新型高效抗菌剂的合成变得越来越重要。在本研究中，通过简便的水热法制备了 ZnO/石墨烯量子点 (GQD) 纳米复合材料，并通过一系列微观和光谱测量进行了表征，包括透射电子显微镜、X 射线光电子能谱、紫外-可见和光致发光光谱。通过最低抑菌浓度和标准平板计数法评估了 ZnO/GQD 纳米复合材料对大肠杆菌的抗菌活性，与 ZnO 和 GQD 分别进行了比较。结果发现，与在环境光下相比，在紫外光照射下活性明显增强。这归因于在紫外光照射下活性氧的生成增强，而电子顺磁共振和荧光显微镜测量表明，膜损伤的贡献较小。研究结果突出了基于半导体纳米粒子和石墨烯衍生物的功能纳米复合材料在开发有效杀菌剂方面的重要性。
