Laboratory MERCI EA3829, University of Rouen, France.
J Tissue Eng Regen Med. 2013 Nov;7(11):901-10. doi: 10.1002/term.1482. Epub 2012 Mar 31.
Haematopoietic stem cells (HSCs) and haematopoietic progenitor cells (HPCs) grow in a specified niche in close association with the microenvironment, the so-called 'haematopoietic niche'. Scaffolds have been introduced to overcome the liquid culture limitations, mimicking the presence of the extracellular matrix (ECM). In the present study the hyaluronic acid scaffold, already developed in the laboratory, has been used for the first time to maintain long-term cultures of CD34⁺ haematopoietic cells obtained from human cord blood. One parameter investigated was the impact on ex vivo survival of CD34⁺ cord blood cells (CBCs) on the hyaluronic acid surface, immobilized with peptides containing the RGD motif. This peptide was conjugated by coating the hyaluronan hydrogel and cultured in serum-free liquid phase complemented with stem cell factor (SCF), a commonly indispensable cytokine for haematopoiesis. Our work demonstrated that these hyaluronan hydrogels were superior to traditional liquid cultures by maintaining and expanding the HPCs without the need for additional cytokines, and a colonization of 280-fold increment in the hydrogel compared with liquid culture after 28 days of ex vivo expansion.
造血干细胞（HSCs）和造血祖细胞（HPCs）在与微环境密切相关的特定龛位中生长，即所谓的“造血龛位”。支架的引入克服了液体培养的局限性，模拟了细胞外基质（ECM）的存在。在本研究中，首次使用实验室中已开发的透明质酸支架来维持从人脐带血中获得的 CD34+造血细胞的长期培养。研究的一个参数是研究 RGD 基序肽固定在透明质酸表面上对 CD34+脐血细胞（CBC）的体外存活的影响。该肽通过涂覆透明质酸水凝胶并在不含血清的液体相中培养来进行缀合，该液体相补充有干细胞因子（SCF），SCF 是造血中常用的不可或缺的细胞因子。我们的工作表明，这些透明质酸水凝胶通过维持和扩增 HPC 而优于传统的液体培养，而无需额外的细胞因子，并且在体外扩增 28 天后，与液体培养相比，水凝胶中的定植增加了 280 倍。
