Apple F S, Benson P, Abraham P A, Rosano T G, Halstenson C E
Hennepin County Medical Center, Minneapolis, MN 55415.
Clin Chem. 1989 Feb;35(2):312-4.
We compared creatinine clearances determined by enzymatic (Kodak Ektachem 700 single-slide, Boehringer Mannheim creatinine PAP) and nonenzymatic (Jaffé, HPLC) methods with glomerular filtration rate measured by inulin clearance in patients with varying degrees of renal function. The Kodak enzymatic assay gave values for creatinine 2 to 3 mg/L higher than the other methods. This resulted in significantly lower creatinine clearances than inulin clearances and creatinine clearances determined by the other methods. However, correlations between all methods for serum and urinary creatinine values and clearances were good. To avoid between assay (enzymatic vs nonenzymatic) discrepancies, manufacturers should agree to an acceptable standard of calibration under the usual conditions used with patients.
我们在不同肾功能程度的患者中，将酶法（柯达Ektachem 700单张试纸，勃林格殷格翰肌酐PAP法）和非酶法（杰氏法、高效液相色谱法）测定的肌酐清除率与菊粉清除率测定的肾小球滤过率进行了比较。柯达酶法测定的肌酐值比其他方法高2至3mg/L。这导致其肌酐清除率显著低于菊粉清除率以及其他方法测定的肌酐清除率。然而，所有方法测定的血清和尿肌酐值及清除率之间的相关性良好。为避免不同检测方法（酶法与非酶法）之间的差异，制造商应就患者常用条件下可接受的校准标准达成一致。
