Finco D R, Tabaru H, Brown S A, Barsanti J A
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Am J Vet Res. 1993 Oct;54(10):1575-8.
Renal clearance procedures were performed on adult mixed-breed dogs with a wide range of renal function. Endogenous creatinine clearance was computed after analyzing plasma and urine for creatinine by use of 2 methods, PAP and kinetic Jaffe. For 20-minute clearance procedures, [14C]inulin clearance was measured simultaneously with endogenous creatinine clearance. For 111 twenty-minute clearance procedures performed on 24 dogs, [14C]inulin clearance was highly correlated with creatinine clearance for both methods of creatinine analysis (R2 = 0.979 for [14C]inulin-PAP; R2 = 0.943 for [14C]inulin-Jaffe). The absolute values for PAP and [14C]inulin clearance were nearly the same (PAP-to-[14C]inulin clearance ratio = 1.03 +/- 0.08), but those for Jaffe clearance were substantially less than those for [14C]inulin clearance (Jaffe-to-[14C]inulin clearance ratio = 0.88 +/- 0.10). The Jaffe-to-[14C]inulin clearance ratio was inversely correlated with degree of renal function (R2 = 0.464), whereas the PAP-to-[14C]inulin clearance ratio was not correlated with degree of renal function (R2 = 0.060). Thus, Jaffe-determined creatinine clearance varied, in relation to [14C]inulin clearance, depending on degree of renal function. In 4 clinically normal dogs, 20-minute and 24-hour sample collections analyzed by use of the PAP method gave clearance values significantly greater, for both periods, than did Jaffe analyses. The PAP-determined creatinine clearance values were less than, but not significantly different from 20-minute exogenous creatinine clearance values determined 10 days after 24-hour collections.(ABSTRACT TRUNCATED AT 250 WORDS)
对具有广泛肾功能的成年杂种犬进行了肾脏清除程序。通过使用两种方法（PAP法和动力学Jaffe法）分析血浆和尿液中的肌酐后，计算内生肌酐清除率。对于20分钟的清除程序，同时测量[14C]菊粉清除率和内生肌酐清除率。在对24只狗进行的111次20分钟清除程序中，对于两种肌酐分析方法，[14C]菊粉清除率与肌酐清除率高度相关（[14C]菊粉-PAP法的R2 = 0.979；[14C]菊粉-Jaffe法的R2 = 0.943）。PAP法和[14C]菊粉清除率的绝对值几乎相同（PAP与[14C]菊粉清除率之比 = 1.03±0.08），但Jaffe法的清除率明显低于[14C]菊粉清除率（Jaffe与[14C]菊粉清除率之比 = 0.88±0.10）。Jaffe与[14C]菊粉清除率之比与肾功能程度呈负相关（R2 = 0.464），而PAP与[14C]菊粉清除率之比与肾功能程度无关（R2 = 0.060）。因此，根据肾功能程度，Jaffe法测定的肌酐清除率相对于[14C]菊粉清除率有所不同。在4只临床正常的狗中，使用PAP法分析的20分钟和24小时样本采集的清除率值在两个时间段均显著高于Jaffe法分析的值。PAP法测定的肌酐清除率值低于24小时采集后10天测定的20分钟外源性肌酐清除率值，但差异不显著。（摘要截短至250字）
