Caglayan Emel Kiyak, Caglayan Kasim, Göcmen Ayse Yesim, Cinar Hamza, Seckin Levent, Seckin Selda, Güngör Bülent, Polat Muhammet Fevzi
Department of Obstetrics and Gynaecology, School of Medicine, Bozok University, Yozgat, Turkey.
Department of Surgery, School of Medicine, Bozok University, Yozgat, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2014 Nov;182:154-9. doi: 10.1016/j.ejogrb.2014.09.023. Epub 2014 Sep 22.
To investigate the protective effect of ethyl pyruvate (EP), an anti-oxidant agent, against ischemia-reperfusion injury in a rat model of ovarian torsion, considering biochemical and histopathological aspects.
Forty Wistar Albino rats were divided into five groups: Group I, sham operation; Group II, ischemia alone, Group III, ischemia-reperfusion; Group IV, ischemia-reperfusion+50 mg/kg EP; and Group V, ischemia-reperfusion+100 mg/kg EP. Through laparotomy, 360° torsion was performed and maintained for 3 h, and detorsion was maintained for a further 3 h. Intraperitoneal EP was given 30 min before the surgical procedure. Ovarian tissues and blood samples were obtained after surgery. Malondialdehyde (MDA) and asymmetric dimethyl arginine (ADMA) levels were measured in both plasma and tissue samples. Tissue sections were evaluated histopatologically. Analysis of variance was used for statistical analyses.
In both serum and tissue samples, ADMA and MDA levels were found to be significantly lower in the EP groups compared with the ischemia alone and ischemia-reperfusion groups (p=0.0001). However, no significant difference was found between groups treated with 50 mg/kg or 100 mg/kg EP. On histopathological evaluation, the total tissue injury score was found to be lower in rats treated with EP. No significant difference was detected between groups treated with 50 mg/kg or 100 mg/kg EP.
Ethyl pyruvate may exert positive effects in ischemia-reperfusion injury in cases of ovarian torsion. However, no difference was detected between 50 mg/kg and 100 mg/kg EP.
考虑到生化和组织病理学方面，研究抗氧化剂丙酮酸乙酯（EP）对大鼠卵巢扭转缺血再灌注损伤的保护作用。
将40只Wistar白化大鼠分为五组：第一组，假手术；第二组，单纯缺血；第三组，缺血再灌注；第四组，缺血再灌注+50mg/kg EP；第五组，缺血再灌注+100mg/kg EP。通过剖腹术进行360°扭转并维持3小时，然后再维持去扭转3小时。在手术前30分钟给予腹腔内EP。手术后获取卵巢组织和血样。测量血浆和组织样本中的丙二醛（MDA）和不对称二甲基精氨酸（ADMA）水平。对组织切片进行组织病理学评估。采用方差分析进行统计分析。
在血清和组织样本中，与单纯缺血组和缺血再灌注组相比，EP组的ADMA和MDA水平显著降低（p=0.0001）。然而，50mg/kg或100mg/kg EP治疗组之间未发现显著差异。组织病理学评估显示，EP治疗的大鼠总组织损伤评分较低。50mg/kg或100mg/kg EP治疗组之间未检测到显著差异。
丙酮酸乙酯可能对卵巢扭转缺血再灌注损伤发挥积极作用。然而，50mg/kg和100mg/kg EP之间未发现差异。
