State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
School of Life Sciences, University of Science and Technology of China , Hefei, 230027, People's Republic of China.
Anal Chem. 2017 Dec 19;89(24):13626-13633. doi: 10.1021/acs.analchem.7b04211. Epub 2017 Dec 1.
Recently, the development of new fluorescent probes for the ratiometric detection of target objects inside living cells has received great attention. Normally, the preparation, modification as well as conjugation procedures of these probes are complicated. On this basis, great efforts have been paid to establish convenient method for the preparation of dual emissive nanosensor. In this work, a functional dual emissive carbon dots (dCDs) was prepared by a one-pot hydrothermal carbonization method. The dCDs exhibits two distinctive fluorescence emission peaks at 440 and 624 nm with the excitation at 380 nm. Different from the commonly reported dCDs, this probe exhibited an interesting wavelength dependent dual responsive functionality toward lysine (440 nm) and pH (624 nm), enabling the ratiometric detection of these two targets. The quantitative analysis displayed that a linear range of 0.5-260 μM with a detection limit of 94 nM toward lysine and the differentiation of pH variation from 1.5 to 5.0 could be readily realized in a ratiometric strategy, which was not reported before with other carbon dots (CDs) as the probe. Furthermore, because of the low cytotoxicity, good optical and colloidal stability, and excellent wavelength dependent sensitivity and selectivity toward lysine and pH, this probe was successfully applied to monitor the dynamic variation of lysine and pH in cellular systems, demonstrating the promising applicability for biosensing in the future.
最近，用于活细胞内目标物的比率检测的新型荧光探针的发展受到了极大关注。通常，这些探针的制备、修饰和缀合过程都很复杂。在此基础上，人们付出了巨大的努力来建立一种方便的双发射纳米传感器的制备方法。在这项工作中，通过一锅水热碳化法制备了一种功能性的双发射碳点（dCDs）。dCDs 在 380nm 激发下表现出两个独特的荧光发射峰，分别位于 440nm 和 624nm。与通常报道的 dCDs 不同，该探针对赖氨酸（440nm）和 pH（624nm）表现出有趣的波长依赖性双响应功能，能够对这两种靶标进行比率检测。定量分析显示，在比率策略下，赖氨酸的线性范围为 0.5-260μM，检测限为 94nM，pH 从 1.5 到 5.0 的变化可以很容易地实现，这是以前用其他碳点（CDs）作为探针没有报道过的。此外，由于其低细胞毒性、良好的光学和胶体稳定性以及对赖氨酸和 pH 的优异的波长依赖性灵敏度和选择性，该探针成功地应用于监测细胞系统中赖氨酸和 pH 的动态变化，为未来的生物传感应用展示了广阔的前景。
