Hou Linqian, Wei Jinya, Xiang Chen, Yang Dezhi, Yang Yaling
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
Yunnan High-Tech Enterprise Development Promotion Association, Kunming, 650021, People's Republic of China.
Mikrochim Acta. 2024 Dec 27;192(1):36. doi: 10.1007/s00604-024-06895-z.
A novel copper and iron doped containing chitosan and heparin sodium carbon dots (CS-Cu,Fe/HS) nanozyme was formulated through a single-step microwave digestion method. CS-Cu,Fe/HS exhibits excellent peroxidase (POD)-like activity and positive charge characteristics, and it can oxidize the negatively charged 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of HO to produce a green compound (ox-ABTS). Furthermore, CS-Cu,Fe/HS enhances electron transfer and provides additional active sites through the valence state transformations of Fe/Fe and Cu/Cu. Interestingly, the POD-like activity of CS-Cu,Fe/HS is inhibited with the introduction of ampicillin (AMP), which may be because the Cu and Fe ions in CS-Cu,Fe/HS form complexes with AMP, leading to changes in the structure or surface properties of the nanozyme, thereby reducing the number of active sites on the nanozyme. Drawing from this, a straightforward and reliable colorimetric sensor was constructed for AMP detection, featuring a linear range of 0.033 to 110 μg/mL and a detection limit as low as 11.6 ng/mL. The proposed detection method for AMP performed well in real samples, with recoveries ranging from 94.8% to 110.2%.
通过单步微波消解方法制备了一种新型的含铜铁掺杂壳聚糖和肝素钠碳点（CS-Cu,Fe/HS）的纳米酶。CS-Cu,Fe/HS表现出优异的过氧化物酶（POD）样活性和正电荷特性，并且在HO存在下能氧化带负电荷的2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）生成绿色化合物（氧化型ABTS）。此外，CS-Cu,Fe/HS通过Fe/Fe和Cu/Cu的价态转变增强电子转移并提供额外的活性位点。有趣的是，氨苄青霉素（AMP）的引入会抑制CS-Cu,Fe/HS的POD样活性，这可能是因为CS-Cu,Fe/HS中的铜离子和铁离子与AMP形成络合物，导致纳米酶的结构或表面性质发生变化，从而减少了纳米酶上的活性位点数量。据此，构建了一种用于AMP检测的简单可靠的比色传感器，其线性范围为0.033至110μg/mL，检测限低至11.6ng/mL。所提出的AMP检测方法在实际样品中表现良好，回收率在94.8%至110.2%之间。
