Zhang Shengrui, Ji Xiaohui, Liu Jin, Wang Qin, Jin Lingxia
Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong, Shaanxi, 723000, China.
Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong, Shaanxi, 723000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15;227:117677. doi: 10.1016/j.saa.2019.117677. Epub 2019 Oct 20.
A new nanoprobe based on yellow-emissive carbon dots (Y-CDs) was developed for sensing full-range intracellular pH values. By using o-phenylenediamine as the raw material, Y-CDs with a quantum yield of 31% were prepared through a one-pot solvothermal carbonization method. The Y-CDs exhibited a distinctive fluorescence emission peak at 570 nm with excitation at 450 nm, showing a very large Stokes shift (120 nm). Notably, the nanoprobe revealed a linear relationship between fluorescence intensity and pH value within the range of pH 4.0 to 8.2, exhibiting the ability of this probe to monitor full-range intracellular pH variations. In addition, the nanosensor possessed excellent photostability and fluorescence reversibility in pH measurements and showed excellent selective detection of the influences of other biological species. The CD-based nanoprobe was successfully used to perform quantitative fluorescence imaging of intracellular pH variation, demonstrating its promise for application in cellular systems.
一种基于黄色发射碳点（Y-CDs）的新型纳米探针被开发用于检测全范围的细胞内pH值。以邻苯二胺为原料，通过一锅溶剂热碳化法制备了量子产率为31%的Y-CDs。Y-CDs在450nm激发下于570nm处呈现出独特的荧光发射峰，具有非常大的斯托克斯位移（120nm）。值得注意的是，该纳米探针在pH 4.0至8.2范围内荧光强度与pH值呈现线性关系，表明该探针具有监测全范围细胞内pH变化的能力。此外，该纳米传感器在pH测量中具有出色的光稳定性和荧光可逆性，并对其他生物物种的影响表现出优异的选择性检测能力。基于碳点的纳米探针成功用于细胞内pH变化的定量荧光成像，证明了其在细胞系统中的应用前景。
