Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 34134, Republic of Korea; Institute of Research and Development, Duy Tan University, Da Nang, Viet Nam.
Department of Biochemistry, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 34134, Republic of Korea.
J Colloid Interface Sci. 2018 Mar 1;513:161-169. doi: 10.1016/j.jcis.2017.11.024. Epub 2017 Nov 10.
Three-dimensional (3D) culture dish patterned with a microwell structure demonstrates a great application potential in biotechnology. This study reports on the easy fabrication of an ordered customizable honeycomb microwell array on the surface of polymer substrates including the commercial Petri dish to create a biological platform for cell culture. The fabrication method is based on a very simple solvent dip-coating technique and the methanol accumulation-induced phase separation in which a binary mixture of chloroform and methanol is used to induce a ternary solution and to guarantee the formation of the ordered pore array on the substrate. The surface topology of the honeycomb substrate is manipulated through varying the experimental conditions; notably, the obtained honeycomb structure is part of the substrate, which reveals an increase in the structure's stability for the practical applications. Honeycomb-structured Petri dish fabricated using this method is applied as a scaffold for cell growth to demonstrate its potential in biomedical applications.
三维（3D）微井结构培养皿在生物技术中具有巨大的应用潜力。本研究报告了一种在聚合物基底（包括商用培养皿）表面上轻松制造有序可定制蜂窝状微井阵列的方法，从而为细胞培养创建了一个生物平台。该制造方法基于非常简单的溶剂浸涂技术和甲醇积累诱导相分离，其中使用氯仿和甲醇的二元混合物来诱导三元溶液，并保证在基底上形成有序的孔阵列。通过改变实验条件来控制蜂窝状基底的表面拓扑结构；值得注意的是，所得到的蜂窝结构是基底的一部分，这提高了结构的稳定性，使其更适用于实际应用。使用这种方法制造的蜂窝状培养皿被用作细胞生长的支架，以展示其在生物医学应用中的潜力。
