School of Water Resources and Environment, China University of Geosciences (Beijing), No. 29 Xueyuan Road, Haidian District, Beijing 100083, China.
School of Water Resources and Environment, China University of Geosciences (Beijing), No. 29 Xueyuan Road, Haidian District, Beijing 100083, China.
Bioresour Technol. 2018 Feb;249:869-879. doi: 10.1016/j.biortech.2017.11.011. Epub 2017 Nov 6.
The NO removal pathway and microorganisms change along with the height of an up-flow W-SPD bioreactor was investigated in this study. Modeling and microbial community analysis were used to analyze the denitrification behavior in W-SPD bioreactor. The results showed that NO removal rate matched for zero-order (R > 0.97) and first-order (R > 0.94) combination Michaelis-Menten kinetics, whereas microbial reaction rate suited for modified logistic model (R > 0.99). The excellent denitrification performance (92.5%-96.4%) and microorganisms' quantity occurred in the middle of W-SPD bioreactor. Moreover, high-throughput sequencing analysis revealed that dominant denitrifiers, carbonaceous compound degrading bacteria and fermentative bacteria co-existed in W-SPD system, which was vital for efficiently sustainable NO removal. Hence, aerobic degradation, heterotrophic denitrification and dissimilatory nitrate reduction to ammonium (DNRA) occurred successively along the water direction in the bioreactor, offering reasonable references for W-SPD bioreactor study and application.
本研究考察了上流式 WSDP 生物反应器中 NO 去除途径和微生物随高度的变化。通过建模和微生物群落分析，对 WSDP 生物反应器中的反硝化行为进行了分析。结果表明，NO 去除率符合零级（R>0.97）和一级（R>0.94）组合米氏动力学，而微生物反应速率适合改进的 logistic 模型（R>0.99）。在 WSDP 生物反应器的中部，反硝化性能（92.5%-96.4%）和微生物数量达到最佳。此外，高通量测序分析表明，优势脱氮菌、碳源化合物降解菌和发酵菌在 WSDP 系统中共存，这对高效可持续的 NO 去除至关重要。因此，在生物反应器中，好氧降解、异养反硝化和异化硝酸盐还原为铵（DNRA）沿水流方向依次发生，为 WSDP 生物反应器的研究和应用提供了合理的参考。
