State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China.
Bioresour Technol. 2020 May;303:122948. doi: 10.1016/j.biortech.2020.122948. Epub 2020 Feb 3.
A heterotrophic nitrifying and aerobic denitrifying fungus was isolated from lake water and identified as Penicillium tropicum strain IS0293. The strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability and could utilize ammonium, nitrite and nitrate as a sole nitrogen source. Batch tests demonstrated that strain IS0293 can remove nitrate using variety of organic carbon compounds as carbon sources. The effect of woodchip leachate collected at different degradation times on denitrification performance of the strain was also investigated. Furthermore, two denitrifying woodchip bioreactors were constructed to assess the bioaugmention of strain IS0293 for nitrate removal from surface water. Results demonstrated that the incubation of strain IS0293 enhanced the nitrate removal efficiency of the bioreactor. In addition, the average effluent TOC content of the bioaugmention bioreactor was 38.22% lower than the control bioreactor. This study would be valuable to develop an effective technology for nitrate-laden surface water under aerobic conditions.
从湖水分离出一株异养硝化和好氧反硝化真菌，并鉴定为青霉属热带种 IS0293 菌株。该菌株表现出高效的异养硝化-好氧反硝化能力，可以利用铵盐、亚硝酸盐和硝酸盐作为唯一氮源。批式实验表明，IS0293 菌株可以利用多种有机碳源去除硝酸盐。此外，还研究了不同降解时间采集的木屑浸出液对菌株反硝化性能的影响。进一步构建了两个反硝化木屑生物反应器，以评估 IS0293 菌株对地表水中硝酸盐去除的生物强化作用。结果表明，IS0293 菌株的孵育提高了生物反应器的硝酸盐去除效率。此外，生物强化生物反应器的平均出水 TOC 含量比对照生物反应器低 38.22%。该研究为在好氧条件下开发有效去除富营养化地表水中硝酸盐的技术提供了参考。
