Cheng Lin, Xia Tian-Song, Shi Liang, Xu Lingyun, Chen Weixian, Zhu Yulan, Ding Qiang
Department of Breast Surgery, The Affiliated Changzhou NO 2. People's Hospital with Nanjing Medical University, 29 Xinglong Lane, Changzhou 213003, China; Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.
Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.
Biochem Biophys Res Commun. 2018 Jan 1;495(1):775-780. doi: 10.1016/j.bbrc.2017.11.081. Epub 2017 Nov 14.
Many natural products have been shown to have inhibitory effects on the metastatic process of various cancers including breast cancer. An active triterpenoid saponin D Rhamnose β-hederin (DRβ-H) from Clematis ganpiniana, has been known induce the apoptosis of breast cancer cells, but the effect of DRβ-H on the metastasis of breast cancer cells is largely unknown. In this study, we demonstrated that a non-cytotoxic concentration of DRβ-H markedly suppressed wound healing migration, migration through the chamber and invasion through the matrigel. In addition, DRβ-H regulated expression of RNPC1, E-cadherin proteins of MDA-MB-231 cells. Furthermore, RNPC1 knockdown decreased the DRβ-H-induced up-regulation of RNPC1 and E-Cadherin in MDA-MB-231 cells. RNPC1 knockdown reduced the anti-metastasis activities of DRβ-H, meaning that the up-rugulation of RNPC1 by DRβ-H is essential for its anti-metastatis activities. These results suggest that DRβ-H might be a potential therapeutic candidate for the treatment of breast cancer metastasis.
许多天然产物已被证明对包括乳腺癌在内的各种癌症的转移过程具有抑制作用。来自威灵仙的一种活性三萜皂苷D鼠李糖β-常春藤皂苷（DRβ-H），已知可诱导乳腺癌细胞凋亡，但DRβ-H对乳腺癌细胞转移的影响在很大程度上尚不清楚。在本研究中，我们证明了非细胞毒性浓度的DRβ-H显著抑制伤口愈合迁移、通过小室的迁移以及通过基质胶的侵袭。此外，DRβ-H调节MDA-MB-231细胞中RNPC1、E-钙黏蛋白的表达。此外，敲低RNPC1可降低DRβ-H诱导的MDA-MB-231细胞中RNPC1和E-钙黏蛋白的上调。敲低RNPC1降低了DRβ-H的抗转移活性，这意味着DRβ-H对RNPC1的上调对其抗转移活性至关重要。这些结果表明，DRβ-H可能是治疗乳腺癌转移的潜在候选药物。
