College of Science, Northeast Agricultural University, Harbin 150030, PR China.
College of Horticulture, Northeast Agricultural University, Harbin 150030, PR China.
Food Chem. 2018 Mar 15;243:26-35. doi: 10.1016/j.foodchem.2017.09.107. Epub 2017 Sep 21.
In this study, the degradation of polysaccharides from blackcurrant (BCP) was investigated. Two low-molecular-weight polysaccharides (DBCP-1, DBCP-2) were obtained using Fe with different concentrations of HO solution. IR spectra showed DBCPs had obvious characteristic peaks of polysaccharides. GC analysis confirmed DBCPs were composed of the same monosaccharide units as BCP but with different molar ratios. NMR analysis indicated DBCPs and BCP had similar glycosidic linkage patterns. The surface area of fragmented structure in DBCPs was reduced compared to BCP, and they had no triple helix structure. The results of bioactivity assays indicated that DBCPs exhibited higher antioxidant, α-amylase and α-glucosidase inhibitory activities than BCP, and the degraded polysaccharides with the lower molecular weight possessed higher bioactivities. These results suggested that Fe-HO degradation did not change the main structure of polysaccharide and the degree of degradation could play a key role in the bioactivities of the polysaccharides.
本研究考察了黑加仑多糖（BCP）的降解。采用 Fe-HO 溶液，得到两种低分子量多糖（DBCP-1、DBCP-2）。IR 光谱显示 DBCPs 具有明显的多糖特征峰。GC 分析证实 DBCPs 由与 BCP 相同的单糖单元组成，但摩尔比不同。NMR 分析表明 DBCPs 和 BCP 具有相似的糖苷键连接模式。与 BCP 相比，DBCPs 中碎片结构的表面积减少，且它们没有三螺旋结构。生物活性测定结果表明，DBCPs 表现出比 BCP 更高的抗氧化、α-淀粉酶和 α-葡萄糖苷酶抑制活性，且分子量较低的降解多糖具有更高的生物活性。这些结果表明，Fe-HO 降解没有改变多糖的主要结构，降解程度可能在多糖的生物活性中起关键作用。
