Morris G P, Beck P L, Herridge M S, Depew W T, Szewczuk M R, Wallace J L
Department of Biology, Queen's University, Kingston, Ontario, Canada.
Gastroenterology. 1989 Mar;96(3):795-803.
We have developed a simple and reproducible rat model of chronic colonic inflammation by the intraluminal instillation of a solution containing a "barrier breaker" and a hapten. Administration of the hapten 2,4,6-trinitrobenzenesulfonic acid (5-30 mg) in 0.25 ml of 50% ethanol as the "barrier breaker" produced dose-dependent colonic ulceration and inflammation. At a dose of 30 mg, trinitrobenzenesulfonic acid/ethanol-induced ulceration and marked thickening of the bowel wall persisted for at least 8 wk. Histologically, the inflammatory response included mucosal and submucosal infiltration by polymorphonuclear leukocytes, macrophages, lymphocytes, connective tissue mast cells, and fibroblasts. Granulomas were observed in 57% of the rats killed 3 wk after induction of inflammation. Langhan's-type giant cells were also observed. Segmental ulceration and inflammation were common. The characteristics and relatively long duration of inflammation and ulceration induced in this model afford an opportunity to study the pathophysiology of colonic inflammatory disease in a specifically controlled fashion, and to evaluate new treatments potentially applicable to inflammatory bowel disease in humans.
我们通过向肠腔内滴注含有“屏障破坏剂”和半抗原的溶液，建立了一种简单且可重复的大鼠慢性结肠炎症模型。以0.25 ml 50%乙醇作为“屏障破坏剂”，给予半抗原2,4,6-三硝基苯磺酸（5 - 30 mg）可产生剂量依赖性的结肠溃疡和炎症。在30 mg的剂量下，三硝基苯磺酸/乙醇诱导的溃疡和肠壁明显增厚至少持续8周。组织学上，炎症反应包括多形核白细胞、巨噬细胞、淋巴细胞、结缔组织肥大细胞和成纤维细胞浸润黏膜和黏膜下层。在炎症诱导3周后处死的大鼠中，57%观察到肉芽肿。还观察到朗汉斯型巨细胞。节段性溃疡和炎症很常见。该模型中诱导的炎症和溃疡的特征及相对较长的持续时间，为以特定可控方式研究结肠炎症性疾病的病理生理学，以及评估可能适用于人类炎症性肠病的新治疗方法提供了机会。
