Preparative fractionation of dextrin by polyethylene glycol: Effects of initial dextrin concentration and pH.

Polyethylene glycol (PEG) was further applied for fractionating dextrin prepared from cassava starch. The initial dextrin concentration and pH of the dextrin solutions were crucially considered in this study with the average molecular-weight dispersity (D) as the index. The results showed that the initial dextrin concentration significantly affected the mass fraction and the molecular weight distribution of each dextrin fraction obtained from gradient PEG precipitation. However, the initial dextrin concentration, which ranged from 0.9% to 3.6%, did not affect the D of the dextrin fractions. Furthermore, the D of the fractions obtained at pHs 4.00, 4.96, 6.00, 6.92, 7.99, 8.96, and 9.91, was 1.364, 1.341, 1.305, 1.286, 1.273, 1.311, and 1.404, respectively, while the dispersity of the parent dextrin was 2.052. These results suggest that the preparative approach, gradient PEG precipitation, is applicable in acidic, neutral, and alkaline environments, and that a weakly alkaline environment is optimal for dextrin fractionation.