Vaseghi Golnaz, Taki Mohamad Javad, Javanmard Shaghayegh Haghjooy
Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Basic Med Sci. 2017 Oct;20(10):1178-1181. doi: 10.22038/IJBMS.2017.9398.
Metastasis is the main cause of death in patients with melanoma. Cannabis-based medicines are effective adjunctive drugs in cancer patients. Tau and Stathmin proteins are the key proteins in cancer metastasis. Here we have investigated the effect of a standardized extract on cell migration and Tau and Stathmin gene expression in the melanoma cell line.
In the treatment group, melanoma (B1617) was treated 48 hr with various concentrations of standardized extract. Cells with no treatment were considered as the control group, then study was followed by Quantitative RT-Real Time PCR assay. Relative gene expression was calculated by the ΔΔct method. Migration assay was used to evaluate cancer metastasis.
Tau and stathmin gene expression was significantly decreased compared to the control group. Cell migration was also significantly reduced compared to controls.
decreased tau and stathmin gene expression and cancer metastasis. The results may have some clinical relevance for the use of cannabis-based medicines in patients with metastatic melanoma.
转移是黑色素瘤患者死亡的主要原因。大麻类药物是癌症患者有效的辅助药物。Tau蛋白和Stathmin蛋白是癌症转移中的关键蛋白。在此，我们研究了一种标准化提取物对黑色素瘤细胞系中细胞迁移以及Tau和Stathmin基因表达的影响。
在治疗组中，用不同浓度的标准化提取物处理黑色素瘤（B1617）48小时。未接受处理的细胞作为对照组，然后通过定量逆转录实时聚合酶链反应（Quantitative RT-Real Time PCR）分析进行研究。通过ΔΔct法计算相对基因表达。迁移分析用于评估癌症转移。
与对照组相比，Tau和Stathmin基因表达显著降低。与对照组相比，细胞迁移也显著减少。
Tau和Stathmin基因表达降低以及癌症转移减少。这些结果可能对于大麻类药物在转移性黑色素瘤患者中的应用具有一定临床意义。
