Luo Xianghui, Dai Yun, Cao Yanfei
Department of Thoracic Surgery, Shaanxi Provincial People's Hospital, Xi'an 710068, P.R. China.
J BUON. 2019 Nov-Dec;24(6):2523-2530.
To investigate the expression of Stathmin and vascular endothelial growth factor C (VEGF-C) in the serum of patients with esophageal cancer (EC) and the diagnostic value of the combined two factors.
88 EC patients (the EC Group) treated in Shaanxi Provincial People's Hospital and 50 healthy people (the Control Group) were selected as subjects of this study. After detecting the levels of Stathmin mRNA and VEGF-C mRNA using real-time quantitative PCR (qRT-PCR) and the expression levels of Stathmin protein and VEGF-C protein using enzyme-linked immunosorbent assay (ELISA), analysis of the relationship between the expression levels of Stathmin mRNA and VEGF-C mRNA in the serum and the clinicopathological features of EC were analyzed.
The levels of Stathmin mRNA and VEGF-C mRNA, Stathmin protein and VEGF-C protein in the serum of the Control Group were lower than those in the EC group (p<0.001). The mRNA and protein expressions of Stathmin and VEGF-C in the serum of EC group were related to lymph node metastasis, clinical stage, grade of differentiation and degree of infiltration (p<0.05). Compared with the combined detection of mRNA, the sensitivity of separate detection of Stathmin mRNA and VEGF-C mRNA was lower, but the specificity of the separate detection of Stathmin mRNA was higher (p<0.05). Compared with the sensitivity of the combined detection of protein, the sensitivity of the separate test of Stathmin protein and VEGF-C protein was lower (p<0.05).
Stathmin and VEGF-C could be used as markers for early diagnosis of EC, and the combined detection of the two factors could improve the sensitivity of diagnosis since the expression levels of mRNAs and proteins of Stathmin and VEGFC in the serum of EC patients were proved to be higher than those of healthy volunteers.
探讨食管癌（EC）患者血清中Stathmin和血管内皮生长因子C（VEGF-C）的表达及其联合检测的诊断价值。
选取陕西省人民医院收治的88例EC患者（EC组）和50例健康人（对照组）作为研究对象。采用实时定量聚合酶链反应（qRT-PCR）检测血清中Stathmin mRNA和VEGF-C mRNA水平，采用酶联免疫吸附测定（ELISA）检测Stathmin蛋白和VEGF-C蛋白表达水平，并分析血清中Stathmin mRNA和VEGF-C mRNA表达水平与EC临床病理特征的关系。
对照组血清中Stathmin mRNA、VEGF-C mRNA、Stathmin蛋白和VEGF-C蛋白水平均低于EC组（p<0.001）。EC组血清中Stathmin和VEGF-C的mRNA及蛋白表达与淋巴结转移、临床分期、分化程度及浸润深度有关（p<0.05）。与mRNA联合检测相比，单独检测Stathmin mRNA和VEGF-C mRNA的敏感性较低，但单独检测Stathmin mRNA的特异性较高（p<0.05）。与蛋白联合检测的敏感性相比，单独检测Stathmin蛋白和VEGF-C蛋白的敏感性较低（p<0.05）。
Stathmin和VEGF-C可作为EC早期诊断的标志物，由于EC患者血清中Stathmin和VEGFC的mRNA和蛋白表达水平高于健康志愿者，联合检测这两个因子可提高诊断敏感性。
