Weiss William M, Mulet-Sierra Aillette, Kunze Melanie, Jomha Nadr M, Adesida Adetola B
Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering, Department of Surgery, University of Alberta, Li Ka Shing Centre for Health Research Innovation, Edmonton, AB Canada.
Department of Orthopedic Surgery and Rehabilitation, Sports Medicine, Reconstruction and Trauma, Texas Tech University Health Sciences Center, Lubbock, TX USA.
NPJ Microgravity. 2017 Nov 10;3:28. doi: 10.1038/s41526-017-0032-x. eCollection 2017.
Simulated microgravity has been shown to enhance cartilaginous matrix formation by chondrocytes and chondrogenesis of mesenchymal stem cells (MSCs). Similarly, coculture of primary chondrocytes with MSCs has been shown as a strategy to simultaneously retain the differentiated phenotype of chondrocytes and enhance cartilaginous matrix formation. In this study, we investigated the effect of simulated microgravity on cocultures of primary human meniscus cells and adipose-derived MSCs. We used biochemical, qPCR, and immunofluorescence assays to conduct our investigation. Simulated microgravity significantly enhanced cartilaginous matrix formation in cocultures of primary meniscus cells and adipose-derived MSCs. The enhancement was accompanied by increased hypertrophic differentiation markers, and , and suppression of hypertrophic differentiation inhibitor, gremlin 1 ().
模拟微重力已被证明可增强软骨细胞形成软骨基质以及间充质干细胞（MSC）的软骨生成。同样，原代软骨细胞与MSC共培养已被证明是一种既能保留软骨细胞分化表型又能增强软骨基质形成的策略。在本研究中，我们调查了模拟微重力对原代人半月板细胞与脂肪来源的MSC共培养的影响。我们使用生化、qPCR和免疫荧光测定法进行研究。模拟微重力显著增强了原代半月板细胞与脂肪来源的MSC共培养中的软骨基质形成。这种增强伴随着肥大分化标志物和的增加，以及肥大分化抑制剂gremlin 1（）的抑制。
