Huschtscha L I, Lucibello F C, Bodmer W F
Imperial Cancer Research Fund, London, United Kingdom.
In Vitro Cell Dev Biol. 1989 Jan;25(1):105-8. doi: 10.1007/BF02624419.
A new method has been developed to count cells "in situ", based on a fluorogenic enzyme assay that measures the activity of alkaline phosphatase. Increasing cell number was shown to correlate closely with alkaline phosphatase activity and this relationship did not change with time in culture. The alkaline phosphatase assay (ALP assay) was able to estimate relative cell numbers over a range from about 10(4) to 5 X 10(5) for many cell types, including Hep-2, a derivative of HeLa, several human colorectal cell lines SW1222, SW837, LS174T and HT29, a normal human diploid cell strain MRC5 and a rodent line NIH-3T3. The ALP assay is rapid and efficient, making it a useful method for studying growth assays.
基于一种测量碱性磷酸酶活性的荧光酶测定法，已开发出一种用于“原位”计数细胞的新方法。细胞数量的增加与碱性磷酸酶活性密切相关，且这种关系在培养过程中不会随时间而改变。碱性磷酸酶测定法（ALP测定法）能够估算多种细胞类型在约10⁴至5×10⁵范围内的相对细胞数量，这些细胞类型包括HeLa的衍生物Hep - 2、几种人类结肠癌细胞系SW1222、SW837、LS174T和HT29、一种正常人二倍体细胞株MRC5以及一种啮齿动物细胞系NIH - 3T3。ALP测定法快速且高效，使其成为研究生长测定的一种有用方法。
